其他技术

Research Tools: Synthetic Biology and Protein Engineering Research Goals: (1)Improve the performance the key proteinssuch as turn-over numberKmsubstrate promiscuitythermostabilityinhibitionetc.by prot ...

This sample preparation method is a direct replacement for the dried droplet technique. It produces more uniform samples than the dried-droplet technique and the crystals are better adhered to the sam ...

James MoviusHahn Lab 1.Proteins are TCA precipitated and washed with acetonethen dried. 2.The CNBr should be brought to room temperature in the hood and used ONLY in the hood.Extremely toxic! 3.Dissol ...

醋酸纤维素薄膜是由醋酸纤维素加工制成的。醋酸纤维素薄膜作为是电泳支持体有以下优点：①电泳后区带界限清晰；②通电时间较短（二十分钟至一小时）；③它对各种蛋白质（包括血清白蛋白，溶菌酶及核糖核酸酶）都几乎完全不吸附，因此无拖尾现象；④对染料也没有吸附，因此不结合的染料能完全洗掉，无样品处几乎完全无色。它的电渗作用虽高但很均一，不影响样品的分离效果，由于醋酸纤维素薄膜吸水量较低，因此必需在密闭的容器中进 ...

Ref. Seed and Sheen Gene 67 271-277 (1988) Normal reaction is Chloramphenicol + Acetyl-CoA -> Acetylchloramphenicol Assay uses butyryl-CoA (more hydrophobic) 14C-Cm+ Bu-CoA->Bu-14C-Cm Km (Cm) 1 ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c55"; ...

Terminal Deoxynucleotidyl Transferase (TdT) is an enzyme that catalyzes the repetitive addition of mononucleotides from dNTPs to the terminal 3´-OH of a DNA initiator accompanied b ...

Material/reagents: • 2X binding buffer: 20 mM Tris pH 7.5 100 mM NaCl 2 mM EDTA ...

There are multiple variations to this protocol, but we find that this one works well in all cases we tested.Reagents: 5X EMSA Buffer: 50mM HEPES (pH 7.9)375 mM KCl12.5 mM MgCl20.5 mM EDTA5 mM DTT15% Ficoll32 P-labeled oligonucleotide probepolydI/dC: 1 mg/ml in TEBSA: 10 mg/ml in TE5% Polyacrylamide gel (30 ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f ...

Wash one 0.45um Millipore nitrocellulose filter (Cat# HAWP01300) in 1ml 0.4M KOH for 1 min; wash in 3ml dH2 O for >1min. Assemble filter apparatus: white plastic bottom support/funnel nitrocellulos ...

Marian Price-Carter, 9/7/00Day 1 : Start overnight cultures in assay medium.Negative control : cells lacking b -galactosidase, such as LT2; positive control : cells with high enzyme activity.Day 2 : Dilute cells 1/100 in fresh medium, grow to mid-log.1 Prepare solutions: Z buffer, phosphate buf ...