RNA原位杂交

NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGENIN dUTPFor the use with FISH (fluorescent in situ hybridization) we always label entire plasmid DNA and do not cut out the insert.1. mix togeth ...

Preparation and purification of biotin-labeled probe for ISHI. Biotin-labeling of plasmid or genomic DNA.Suggested chemicals: Nick Translation Kit Enzo Diagnostics Cat. No. 42803. Prepare the followin ...

Hiro Hirai's BAC-FISH ProtocolOverviewThis protocol is a modification of the standard FISH protocol published by Hiro Hirai and Phil LoVerde in Parasitology Today (1995 11(8) p 310-314) that has been ...

Anderson Lab In Situ Hybridization ProtocolsMarch 1995 These protocols describe non-radioactive methods for in situ hybridization on frozen sections whole mount embryos and on cultured cells. They hav ...

FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ON HUMAN CHROMOSOMES AND INTERPHASE NUCLEIDept of Medical GeneticsUniversity Hospital GentB-9000 Gent BelgiumSLIDE PRETREATMENT a. RNase pretreatment. incubat ...

I. General ConsiderationsIn situ hybridization is a powerful technique which can be very informative. It should be however carefully controlled since it can lead to artefacts. One of the most importan ...

I. IntroductionMost current methods of measuring gene expression rely on averaging many cellular responses or artificial amplification steps to reach a detectable threshold of signal. In contradistinc ...

IN SITU HYBRIDIZATION ON FROZEN SECTIONSProtocol by Josiah N. Wilcox Ph.D. Remove slides from freezer thaw for 5 min. at 55°C. Fix 10 min. in 4% paraformaldehyde 4°C. Wash 5 min. in 0.5x SSC RT. Immer ...

In Situ HYBRIDIZATION TO TISSUE SECTIONS(Kornberg et al. Cell 40:45 1985))PARAFFIN SECTIONSPreparation of embryos (¾10 hr) for paraffin sections Wash embryos off collection plates onto a nylon screen ...

I. General considerations: In situ hybridization of yeast cells is almost identical to mammalian cells except that the cell wall has to be removed by spheroplasting the cells prior to hybridization. T ...

In Situ Hybridization Using Digoxigenin Labeled Probes The Cytogenetics Core performs polytene chromosome in situ hybridizations to polytene chromosomes to serve the needs of the BDGP's various projec ...

June 1997 - optimized by N. Kertesz L.Leyns & E. De RobertisPlease cite: Belo J.A Bouwmeester T. Leyns L.Kertesz N. Gallo M. Follettie M. and De Robertis E.M. "Cerberus-like is a secreted factor with ...

mRNA In Situ Hybridization with Nick-Translated ProbesCells are fixed with freshly made 4% formaldehyde in PBS pH 7.4 for 15 min at room temperature. All solutions should be made in Molecular Biology ...

PREPARATION OF STOCK SOLUTIONS FOR FISH note: use for all preparations deionised bidestilled and sterilized waterRNase A (10 mg/ml)-dissolve 10 mg in 1ml of 10 mM Tris-HCl (pH 7.5) and 15 mM NaCl-put ...

Protein-DNA Telomere FISHFIX AND PROTEIN STRAIN:Fix cells in 4% paraformaldhyde (diluted in PBS) for 10’ at room temp. Wash cells with PBS +0.2% Tween20 Permeabilize cells with 0.5% Triton X-100 for 1 ...

RNA in situ protocol for DNA and RNA probes This procedure was adapted from one originally described by Tautz and Pfeifle (1989). It makes use of digoxigenin labelled probes and alkaline phosphatase-c ...

TISSUE PREPARATION FOR IN SITU HYBRIDIZATIONJosiah N. Wilcox Harvest tissue and rinse in PBS or saline. Immerse tissue in 4% paraformaldehyde/0.1M sodium phosphate buffer pH7.4 (recipe follows) at 4°C ...

Hiro Hirai's PRINS ProtocolOverviewThe PRINS (Primed in situ) technique uses a specific primer dNTPs with Dig-11-dUTP and DNA polymerase to perform a primer extension reaction on a chromosome preparat ...

A PDF file of our non-radioactive in situ hybridisation protocol version 08.03.2004. 点击浏览该文件 ...

一、核酸分子杂交技术1961年Hall开拓了液相核酸杂交技术的研究，其基本原理是利用核酸分子单链之间有互补的碱基顺序，通过碱基对之间非共价键的形成，出现稳定的双链区，形成杂交的双链。自此以后，由于分子生物学技术的迅猛发展，特别是70年代末到80年代初，分子克隆、质粒和噬菌体DNA的构建成功，核酸自动合成仪的诞生，大大丰富了核酸探针的来源，新的核酸分子杂交类型和方法不断涌现。按其作用方式可大致分为固 ...