DNA基础知识

Recovery of DNA from LMP (Low Melting Point) Agarose Gel1. Separate DNA fragments through an LMP agarose gel containing ethidium bromide (0.5 microgram/ml). 2. Detect DNA by irradiating the gel with l ...

Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium acetate pH 5.2 (final con ...

Improved Alcohol Precipitation of DNAECKDescription========This method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley 1985). It is a useful way to avoid coprecipitation of ...

Improved Alcohol Precipitation of DNAECKDescription========This method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley 1985). It is a useful way to avoid coprecipitation of ...

Sephadex G-50 spun column purificationSpin column purification can be used to change buffers without a concomitant change in solution volume to remove protein contaminants or to purify plasmid DNA sui ...

1. Prepare or obtain Buffered phenol pH 8. Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation. This also identifies the organic phase as yellow-colored.2. Combine DNA sample with an equal volum ...

Preparation of linear polyacrylamide used as carrier in ethanol precipitation of nucleic acidsLinear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with eth ...

DNA Extraction MethodsPhenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl 10 mM EDTA 50 mM Tris-HCl pH 8). 2. Incubate The sand fly ho ...

DNA Extraction MethodsPhenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl 10 mM EDTA 50 mM Tris-HCl pH 8). 2. Incubate The sand fly ho ...

Rapid Method for Preparation of Genomic DNA from P. aeruginosa Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37° ...

Rapid Method for Preparation of Genomic DNA from P. aeruginosa Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37° ...

Preparation of Sonicated Salmon Sperm DNAProcedure:1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps weigh 0.25 g/50 ml conical and add 50 ml/conical of 0.02 M Tris pH 7.6. Allow to ...

This is a modification of a salting out procedure as described by Miller et al. (1988) evaluated at the DNA Laboratory Medical School Malta. When analysed by spectrophotometry 95% of extracted genomic ...

This is a modification of a salting out procedure as described by Miller et al. (1988) evaluated at the DNA Laboratory Medical School Malta. When analysed by spectrophotometry 95% of extracted genomic ...

DNA From Whole Blood for PCR(from Higuchi R. (1989) Amplifications 2: 1-3)Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube. Expel blood immediately into a 1.5 ml ...

Extraction of genomic DNA from whole bloodAuthor: Laura-Lee BoodramSource: Laura-Lee Boodram Department of Life Sciences The University of the West IndiesAbstract: The protocol is simple and fairly ra ...

DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS - make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sections for the DNA isolation- put sections in a falcon tube containing ...

ES CELL DNA EXTRACTION: TUBE METHOD Protocol for extracting DNA from ES Cells starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA. NOTE- THIS TAKES A LOT OF ...

macerate tissue in Eppendorf tube without butter at RT add 400 ml extraction buffer vortex for 4 sec leave sample at RT until other samples are ready ( 1 h) spin in microfuge for 1 min transfer 300 ml ...

Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. Mix well. Most DNA solutions can be vortexed for 10 sec except for high ...