数据库

Abstract Table of Contents Materials Literature Cited Abstract The principle of cloning utilizes hybridization of single?stranded DNA probes to denatured DNA fixed on membranes to detect the identical DNA molecules present in a large popul

Abstract Table of Contents Figures Literature Cited Abstract Synthetic nucleic acids are produced routinely for a wide variety of applications, including biological and chemical research, and diagnostic or therapeutic applications. To ensu

Abstract Table of Contents Figures Literature Cited Abstract Database sequence similarity searching is carried out thousands of times each day by researchers worldwide and has become a very valuable tool. Over the years, a number of algori

Abstract Table of Contents Figures Literature Cited Abstract This unit outlines a variety of methods by which DNA sequences can be manipulated by computers. Procedures for entering sequence data into the computer and assembling raw sequenc

Abstract Table of Contents Figures Literature Cited Abstract This unit describes the NCBI's Entrez database browser. Entrez integrates DNA and protein sequence data, threedimensional structures, and taxonomic information with its associate

Abstract Table of Contents Figures Literature Cited Abstract With the explosion of sequence and structural information available to researchers, the field of bioinformatics is playing an increasingly large role in the study of fundamental

Abstract Table of Contents Figures Literature Cited Abstract This brief appendix serves as a guide for the analysis of functional motifs in proteins. Several database search engines that can be accessed via the World Wide Web are described

Abstract Table of Contents Materials Figures Literature Cited Abstract Specific sequences in RNA preparations can be detected by blotting and hybridization analysis using techniques very similar to those

Abstract Table of Contents Figures Literature Cited Abstract This unit introduces readers to some of the more commonly used techniques for gene identification. The author discusses the general problem behind accurately predicting genes in

Abstract Table of Contents Figures Literature Cited Abstract DNA microarrays or chips can be used to simultaneously monitor the expression levels and/or genotypes of thousands of genes. The application of these techniques heralds a new era

Abstract Table of Contents Materials Figures Literature Cited Abstract The ability to construct comprehensive gene expression profiles comprising hundreds to thousands of genes whose RNA levels are monito

Abstract Table of Contents Figures Literature Cited Abstract Nucleic acid array technology refers to the use and fabrication of arrays containing thousands of nucleic acid samples bound to solid substrates such as glass microscope slides o

Abstract Table of Contents Materials Figures Literature Cited Abstract cDNA Library Construction from Single Cells (Phillip R. Kramer, National Institute of Neuronal Disorders and Stroke, NIH, Bethesda, M

Abstract Table of Contents Literature Cited Abstract The International Union of Pure and Applied Chemistry (IUPAC) and the International Union of Biochemistry and Molecular Biology (IUBMB) have established standards for representing nucleic acids and amino acids with single

Abstract Table of Contents Figures Literature Cited Abstract TreeView provides a simple way to view the phylogenetic trees produced by a range of programs, such as PAUundefined, PHYLIP, TREE?PUZZLE, and ClustalX. While some phylogenetic programs

Abstract Table of Contents Figures Literature Cited Abstract MZEF (Michael Zhang's Exon Finder) was designed to help identify one of the most important classes of exons, i.e. the internal coding exons, in human genomic DNA sequences. It is

Abstract Table of Contents Materials Figures Literature Cited Abstract The Framesearch algorithm includes the possibility of a frameshift error in its alignment algorithm, and therefore can find alignment

Abstract Table of Contents Materials Figures Literature Cited Abstract The Clustal programs are widely used for carrying out automatic multiple alignment of nucleotide or amino acid sequences. The most fa

Abstract Table of Contents Materials Figures Literature Cited Abstract Blocks are ungapped multiple alignments of related protein sequence segments that correspond to the most conserved regions of the pro

Abstract Table of Contents Figures Literature Cited Abstract GlimmerM is a eukaryotic gene finder that has been used in the annotation of the genomes of Plasmodium falciparum (the malaria parasite), the model plant Arabidopsis thaliana, Or