Overview of Purification and Analysis of Synthetic Nucleic Acids

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Abstract
Table of Contents
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Literature Cited

Abstract

Synthetic nucleic acids are produced routinely for a wide variety of applications, including biological and chemical research, and diagnostic or therapeutic applications. To ensure an adequate level of quality and purity, rapid and convenient analytical methods are necessary. This unit discusses basic principles to guide in the selection of appropriate purification and analysis protocols.

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Selecting Analysis/Purification Methods
Post‐Synthesis Procedures for Oligonucleotides
Mixed‐Base Oligonucleotides
Modified Oligonucleotides
Literature Cited
Figures
Tables

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Materials

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Figures

Figure 10.3.1 Absorbance spectra of four natural deoxynucleosides, dA, dC, dG, and T in aqueous solution at pH of 7.0.

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Figure 10.3.2 Absorbance spectra of an 18‐nt oligonucleotide 5′ TCA CAG TCT GAT CTC GAT 3′ in 0.1 M TEAA, pH 7.0.

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Literature Cited

Literature Cited
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