• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Overview of Purification and Analysis of Synthetic Nucleic Acids

        互联网

        557
        • Abstract
        • Table of Contents
        • Figures
        • Literature Cited

        Abstract

         

        Synthetic nucleic acids are produced routinely for a wide variety of applications, including biological and chemical research, and diagnostic or therapeutic applications. To ensure an adequate level of quality and purity, rapid and convenient analytical methods are necessary. This unit discusses basic principles to guide in the selection of appropriate purification and analysis protocols.

             
         
        GO TO THE FULL PROTOCOL:
        PDF or HTML at Wiley Online Library

        Table of Contents

        • Selecting Analysis/Purification Methods
        • Post‐Synthesis Procedures for Oligonucleotides
        • Mixed‐Base Oligonucleotides
        • Modified Oligonucleotides
        • Literature Cited
        • Figures
        • Tables
             
         
        GO TO THE FULL PROTOCOL:
        PDF or HTML at Wiley Online Library

        Materials

         
        GO TO THE FULL PROTOCOL:
        PDF or HTML at Wiley Online Library

        Figures

        •   Figure 10.3.1 Absorbance spectra of four natural deoxynucleosides, dA, dC, dG, and T in aqueous solution at pH of 7.0.
          View Image
        •   Figure 10.3.2 Absorbance spectra of an 18‐nt oligonucleotide 5′ TCA CAG TCT GAT CTC GAT 3′ in 0.1 M TEAA, pH 7.0.
          View Image

        Videos

        Literature Cited

        Literature Cited
           Andrus, A. 1992a. AutoAnalysis: Trityl monitoring of DNA synthesizer operation by conductivity. Research News, Applied Biosystems, Foster City, California.
           Andrus, A. 1992b. Evaluating and Isolating Synthetic Oligodeoxynucleotides. Applied Biosystems, Foster City, California.
           Andrus, A. 1992c. Oligodeoxynucleotide analysis by gel capillary electrophoresis. In Methods: A Companion to Methods in Enzymology, Vol. 4 (J. Wiktorowicz ed.) pp. 213‐226. Academic Press, San Diego.
           Andrus, A. 1994. Gel‐capillary electrophoresis analysis of oligonucleotides. In Protocols for Oligonucleotide Conjugates (S. Agrawal, ed.) pp. 277‐300. Humana Press, Totowa, N.J.
           Beaucage, S.L. and Iyer, R.P. 1992. Advances in the synthesis of oligonucleotides by the phosphoramidite approach. Tetrahedron 48:2223‐2311.
           Bellenson, J. and Smith, A.J. 1992. Increasing DNA synthesizer throughput via off‐instrument cleavage and deprotection. BioTechniques 12:219‐220.
           Boal, J.H., Wilk, A., Harindranath, N., Max, E., Kempe, T., and Beaucage, S.L. 1996. Cleavage of oligodeoxyribonucleotides from controlled‐pore glass supports and their rapid deprotection by gaseous amines. Nucleic. Acids Res. 24:3115‐17.
           Caruthers, M.H. and Beaucage, S.L. 1983. Phosphoramidite compounds and processes. United States Patent 4,415,732.
           Caruthers, M.H. and Matteucci, M.D. 1984. Process for preparing polynucleotides. United States Patent 4,458,066.
           Ellington, A. 1995. Synthesis and purification of oligonucleotides. In Current Protocols in Molecular Biology, Vol.1 John Wiley & Sons (F. Ausubel et al, eds.) Unit 2.11., New York.
           Kaufman, J., Le, M., Ross, G., Hing, P., Budiansky, M., Yu, E., Campbell, E., Yoshimura, V., Fitzpatrick, V., Nadimi, K., and Andrus, A. 1993. Trityl monitoring of automated DNA synthesizer operation by conductivity: A new method of real‐time analysis. BioTechniques 14:834‐839.
           McCollum, C. and Andrus, A. 1991. An optimized polystyrene support for rapid, efficient oligonucleotide synthesis. Tetrahedron Lett. 32:4069‐4072.
           Reynolds, T.R. and Buck, G.A. 1992. Rapid deprotection of synthetic oligonucleotides. BioTechniques 12:518‐521.
           Theisen, P., McCollum, C., and Andrus, A. 1993. N‐6‐Dialkylformamidine‐2′‐deoxyadenosine phosphoramidites in oligodeoxynucleotide synthesis. Rapid deprotection of oligodeoxynucleotides. Nucleosides Nucleotides 12:1033‐1046.
        GO TO THE FULL PROTOCOL:
        PDF or HTML at Wiley Online Library
         
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序