数据库

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method for interstrand cross?linking between a furan?modified oligonucleotide and its unmodified complement. Th

Abstract Table of Contents Materials Figures Literature Cited Abstract BioID is a unique method to screen for physiologically relevant protein interactions that occur in living cells. This technique harne

Abstract Table of Contents Materials Figures Literature Cited Abstract The resurgence of pulsed electron paramagnetic resonance (EPR) in structural biology centers on recent improvements in distance measu

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the use of cannabinoid radioligands in competitive binding assays for determining affinity parameters (IC50, Ki)

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes an in vitro assay for characterization of the picrotoxin site of GABAA receptors in rat brain membranes using vario

Abstract Table of Contents Materials Figures Literature Cited Abstract Described in this unit are ligand?binding assays for GABAA, GABAB, and the homomeric ? GABAA (formerly GABAC) receptor recognition site

Abstract Table of Contents Materials Figures Literature Cited Abstract This protocol describes the preparation of O4?thymidine?alkylene?O4?thymidine dimer bis?phosphoramidites and precursors for incorporati

Abstract Table of Contents Materials Figures Literature Cited Abstract Triazole?backbone oligonucleotides are macromolecules that have one or more triazole units that are acting as a backbone mimic. Triaz

Abstract Table of Contents Materials Figures Literature Cited Abstract The knocksideways system inactivates proteins by using a small molecule to trap them onto mitochondria. It is typically ?3 to 4 order

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes several assays for detecting several kinds of DNA damage (strand breaks, internal crosslinking, DNA/protein cross

Abstract Table of Contents Figures Literature Cited Abstract Insights into the 3D?structure of a protein have proven useful during experiment design. Experimentally elucidated structures are often not available, but comparative protein mod

Abstract Table of Contents Figures Literature Cited Abstract This unit addresses how to predict the tertiary structure of a protein from its amino acid sequence using computational methods. Three types of prediction methods??homology model

Abstract Table of Contents Figures Literature Cited Abstract With the explosion of sequence and structural information available to researchers, the field of bioinformatics is playing an increasingly large role in the study of fundamental

Abstract Table of Contents Figures Literature Cited Abstract This unit describes procedures developed for predicting protein structure from the amino acid sequence. The first of the four sections is an overview and brief history of structu

Abstract Table of Contents Figures Literature Cited Abstract Hydrophobic interactions are a major force in protein folding and numerous hydropathy scales have been developed to quantify the relative hydrophobicity of the amino acids. Hydro

Abstract Table of Contents Materials Figures Literature Cited Abstract This method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti?sense primers directed ag

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit presents a method for the amplification of poly(A)+ mRNA extracted from the cytoplasm of a single cell. After cDNA is synthes

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit contains protocols for expression cloning in mammalian cells. Either calcium phosphate? or liposome?mediated transfection o

Abstract Table of Contents Materials Literature Cited Abstract Expression cloning requires a representative cDNA or genomic DNA library and a host organism in which the cloned genes can be transcribed and/or translated. It likewise require

Abstract Table of Contents Materials Figures Literature Cited Abstract Of the many proteins that are known to be involved in neuronal signaling, one family of gene products, collectively referred to as th