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Abstract Table of Contents Materials Figures Literature Cited Abstract Streptavidin affinity purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors,

Abstract Table of Contents Figures Literature Cited Abstract The atomic force microscope (AFM) has the unique capability of imaging biological samples with molecular resolution in buffer solution. In addition to providing topographical ima

Abstract Table of Contents Figures Literature Cited Abstract The tumor necrosis factor (TNF) superfamily consists of a wide variety of cell?bound and secreted proteins that regulate numerous cellular processes. In particular, TNF?family pr

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a strategy for attaching two mannose and two galactose residues to an oligonucleotide. This conjugation can be pe

Abstract Table of Contents Materials Figures Literature Cited Abstract Fluorescence is an extremely powerful tool in modern biology, physics, and chemistry laboratories. This unit focuses on methods that

Abstract Table of Contents Materials Figures Literature Cited Abstract The advent of genetically encoded FRET?based kinase activity reporters has ushered in a new era of signal transduction research. Such

Abstract Table of Contents Materials Figures Literature Cited Abstract G protein?coupled receptors (GPCRs) and their signal transductions are important for both physiological and pathological processes in

Abstract Table of Contents Materials Figures Literature Cited Abstract Methylation?sensitive single?molecule analysis of chromatin structure is a high?resolution method for studying nucleosome positioning

Abstract Table of Contents Materials Figures Literature Cited Abstract Understanding of transcriptional regulation has advanced in recent years in part due to development of technologies which allow deter

Abstract Table of Contents Materials Figures Literature Cited Abstract Chromatin Interaction Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis

Abstract Table of Contents Materials Figures Literature Cited Abstract A method is described for the quantitative analysis of protein?protein interactions using the flow cytometry protein interaction assa

Abstract Table of Contents Materials Figures Literature Cited Abstract Glycosylation is an important post?translational modification that influences many biological processes critical for development, nor

Abstract Table of Contents Materials Figures Literature Cited Abstract The biosensors developed in the authors' laboratory have been based on different designs, each imparting specific strengths and weakn

Abstract Table of Contents Materials Figures Literature Cited Abstract Members of the G protein?coupled receptor (GPCR) superfamily have been shown to homo? and hetero?oligomerize both in vitro and in viv

Abstract Table of Contents Materials Figures Literature Cited Abstract Chromatin structure is influenced by post?translational modifications on histones, the principal basic protein component of chromatin

Abstract Table of Contents Literature Cited Abstract In the mid? to late 1990s, because of the drug discovery paradigm shift from phenotypic screens to combinatorial chemistry and high?throughput screening, the physicochemical properties of exploratory drug molecules displa

Abstract Table of Contents Figures Literature Cited Abstract Nucleotide analogues represent a major class of anti?cancer and anti?viral drugs, and provide an extremely powerful tool for dissecting the mechanisms of DNA and RNA polymerases.

Abstract Table of Contents Materials Figures Literature Cited Abstract Measuring the rate and extent of radioligand binding provides information on the number of binding sites, and their affinity and acce

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes ChIP?Seq methodology, which involves chromatin immunoprecipitation (ChIP) followed by high?throughput sequencing

Abstract Table of Contents Materials Figures Literature Cited Abstract Genetically encodable FRET?based kinase activity reporters (KARs) enable real?time monitoring of kinase activity dynamics in living c