数据库

Abstract Table of Contents Materials Figures Literature Cited Abstract Peptide?oligonucleotide conjugates incorporating all the nucleobases and trifunctional amino acids are obtained by Diels?Alder reacti

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method allowing RNA visualization in live cells. The method is based on fluorescent protein complementation reg

Abstract Table of Contents Materials Figures Literature Cited Abstract SPOT arrays consist of synthesized peptides 12? to 18?amino acids long, with overlapping sequences that cover the entire sequence of

Abstract Table of Contents Materials Figures Literature Cited Abstract The detailed synthetic protocol for a 4?selenothymidine phosphoramidite and its use to prepare modified oligonucleotides is described

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit addresses the applications of fluorescence microscopy and quantitative imaging to study multiple physiological variables of

Abstract Table of Contents Materials Figures Literature Cited Abstract The classical yeast two?hybrid system and its modifications have been successfully used over the past decade to investigate interacti

Abstract Table of Contents Figures Literature Cited Abstract The Molecular Interactions Database (MINT) is a relational database designed to store information about protein interactions. Expert curators extract the relevant information fro

Abstract Table of Contents Figures Literature Cited Abstract This unit offers a general overview of several techniques that have been developed for inferring functional and/or protein?protein interaction networks. The majority of these use

Abstract Table of Contents Materials Figures Literature Cited Abstract Fluorescence resonance energy transfer (FRET) is a fluorescence microscopy technique suitable for live cells and capable of detecting

Abstract Table of Contents Materials Figures Literature Cited Abstract Investigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynam

Abstract Table of Contents Materials Figures Literature Cited Abstract Labeling nucleic acids with radioisotopes, fluorophores, biotin, or digoxigenin enables their detection and analysis. When designing

Abstract Table of Contents Materials Figures Literature Cited Abstract Native gels enable the analysis of protein complexes on a proteome?wide scale in a single experiment. The proto

Abstract Table of Contents Materials Figures Literature Cited Abstract The Cu(I)?catalyzed alkyne?azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini?DNA duplexes. The reaction is

Abstract Table of Contents Materials Figures Literature Cited Abstract The sequential functionalization of long?chain alkylamine controlled?pore glass (CPG) with a 3?hydroxypropyl?(2?cyanoethyl)thiophosphor

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes an acceptor?sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acc

Abstract Table of Contents Materials Figures Literature Cited Abstract The yeast two?hybrid system, or interaction trap, is one of the most versatile methods available with which to identify and establish

Abstract Table of Contents Materials Figures Literature Cited Abstract One of the most completely validated processes involved in secondary tissue damage following acute brain or spinal cord injury and in

Abstract Table of Contents Materials Figures Literature Cited Abstract In recent years, several methods have been developed to analyze protein?protein interactions under native conditions. One of them, ta

Abstract Table of Contents Materials Figures Literature Cited Abstract Useful information about glycoconjugates can be obtained by labeling their aglycone (noncarbohydrate) portions?e.g., labeling protein

Abstract Table of Contents Materials Figures Literature Cited Abstract General methods for the synthesis of nucleosides modified with borane clusters and metallacarborane complexes are presented. These in