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Abstract Table of Contents Figures Literature Cited Abstract This overview unit focuses on the basics of fluorescence and of the FRET phenomenon, and on methods for detecting FRET and data interpretation. FRET is very versatile and there a

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the process and analysis of affinity selecting bacteriophage M13 from libraries displaying combinatorial peptides

Abstract Table of Contents Materials Figures Literature Cited Abstract Liquid chromatography techniques have been successfully coupled with mass spectrometers to provide a robust method for the identifica

Abstract Table of Contents Literature Cited Abstract The analytical ultracentrifuge is a high speed centrifuge with an optical system allowing observation of the concentration of macromolecules as a function of radius and time. In sedimentation velocity experiments,relativ

Abstract Table of Contents Figures Literature Cited Abstract Various types of minor groove binders have been attached to synthetic oligodeoxynucleotides, and the interactions of these conjugates (MB?ODNs) with DNA are reviewed here. MB?ODN

Abstract Table of Contents Materials Figures Literature Cited Abstract Described here are several computational procedures for the analysis of electrostatic interactions in molecular complexes, all based

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a Scintillation Proximity Assay (SPA) for the measurement of ligand binding to melanocortin receptors (MCRs) usin

Abstract Table of Contents Materials Figures Literature Cited Abstract The preparation of two reversible biotinylation phosphoramidites and their application in labeling and affinity purification of synth

Abstract Table of Contents Materials Figures Literature Cited Abstract 5??Iodinated oligodeoxyribonucleotides readily react with 3??phosphorothioated DNA in the presence of a complementary template to yie

Abstract Table of Contents Materials Figures Literature Cited Abstract The conjugation of maleimide reporter groups to 5??diene?modified oligonucleotides via aqueous Diels?Alder cycloaddition is described

Abstract Table of Contents Materials Figures Literature Cited Abstract A facile method for preparation of uridine 2??carbamate derivatives based on reaction of 3?,5??disilyl?protected uridine with 1,1??ca

Abstract Table of Contents Materials Figures Literature Cited Abstract Protein?protein interactions play a critical role in biology. Disrupting a specific protein?protein interaction and studying the resu

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes procedures for performing competition binding assays with neurotensin receptor subtypes 1 and 2 (NTS1 and NTS2).

Abstract Table of Contents Figures Literature Cited Abstract In recent years, optical evanescent wave biosensors have been used to characterize protein?protein interactions, including determination of equilibrium binding constants and bimo

Abstract Table of Contents Materials Figures Literature Cited Abstract The aim of this unit is to provide a method for the identification of new protein?protein interactions. Pull?down experiments with GS

Abstract Table of Contents Materials Figures Literature Cited Abstract CGRP receptor binding may be measured using homogenates of cell membranes or intact cells. Here a microcentrifugation?based assay is

Abstract Table of Contents Materials Figures Literature Cited Abstract Chromatin immunoprecipitation (ChIP) is a powerful and widely applied technique for detecting the association of individual proteins

Abstract Table of Contents Materials Figures Literature Cited Abstract Hydroxyl?radical protein footprinting is a straightforward and direct method to map protein sites involved in macromolecular interact

Abstract Table of Contents Materials Literature Cited Abstract This unit describes the combination of chromatin immunoprecipitation (ChIP) with microarray hybridization to determine the genome?wide occupancy profile of a DNA?associated pro

Abstract Table of Contents Figures Literature Cited Abstract Cytokines are key regulatory molecules that serve as critical mediators of cell?to?cell communication. They play a central role in immune function and are important molecular tar