数据库

Abstract Table of Contents Materials Figures Literature Cited Abstract The mammalian tachykinin peptides are a family of neuropeptides characterized by a common C?terminal amino acid sequence of the form

Abstract Table of Contents Materials Literature Cited Abstract The ubiquitous distribution of EAA receptors results from the fact that EAA?mediated neurotransmission is the most prevalent type of fast, chemically?mediated communication in

Abstract Table of Contents Materials Figures Literature Cited Abstract Corticotropin?releasing factor (CRF) and its receptors play a major role in regulating an organism's response to physical, emotional

Abstract Table of Contents Materials Figures Literature Cited Abstract Cholecystokinin (CCK) and gastrin are structurally related peptide hormones synthesized and secreted by endocrine cells in the gastri

Abstract Table of Contents Materials Figures Literature Cited Abstract Neuropeptide Y (NPY), peptide YY (PYY), and pancreatic polypeptide (PP) constitute a trio of structurally related peptides that elici

Abstract Table of Contents Materials Figures Literature Cited Abstract Angiotensin II (Ang II) is the primary mediator of the renin angiotensin system and acts primarily as a circulating hormone, but may

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the preparation and applications of porphyrinic sensors for quantitative measurement of nitric oxide (NO) in sing

Abstract Table of Contents Materials Figures Literature Cited Abstract Nitric oxide (NO) is a key biosignaling molecule produced in both peripheral tissues and the central nervous system by a family of en

Abstract Table of Contents Materials Literature Cited Abstract cAMP acts as an intracellular mediator of hormone action and the importance of accurate quantitative determination of cAMP levels in cells and tissues is widely recognized. The

Abstract Table of Contents Materials Figures Literature Cited Abstract Ca2+, Na+, K+, and Mg2+ have a central role in neuronal excitability. The concentration of these cations in the cytoplasm of neurons

Abstract Table of Contents Materials Figures Literature Cited Abstract In this unit, protocols are described for biochemical and optical techniques that have been used by investigators to measure ligand?g

Abstract Table of Contents Materials Figures Literature Cited Abstract The availability of clonal cell lines for norepinephrine, dopamine, and serotonin transporters allows the characterization of drug in

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the use of a Cytosensor microphysiometer for functional characterization of an agonist and antagonist to a G prot

Abstract Table of Contents Materials Figures Literature Cited Abstract The use of filtration assays is often unsuitable for radioligands with rapid dissociation rates. Rapid dissociation may result in the

Abstract Table of Contents Materials Figures Literature Cited Abstract The protocols in this unit describe methods for measuring radioligand binding to sites on the GABA?A receptor complex. This system wa

Abstract Table of Contents Materials Figures Literature Cited Abstract Solid?phase synthesis of 3??aminoalkyl, 3??thioalkyl, and 3??polyethyleneglycol are described. The first two are important as specifi

Abstract Table of Contents Figures Literature Cited Abstract Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid?phase oligonucleotide synthesis supports. However, 3?olig

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit gives protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protoc

Abstract Table of Contents Figures Literature Cited Abstract Reporter and conjugate groups can be added directly to the 5? terminus of oligonucleotides by appropriate modification. Conjugate groups can be used to increase the affinity of c

Abstract Table of Contents Materials Figures Literature Cited Abstract Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made ea