实验基础

It is well established that dendritic cells (DC) exhibit different phenotypes and functions as they progress down the developmental pathway toward interdigitating DC that stimulate T cells in the secondary lymphoid tissue (1). In the blood, two populations of DC can be identified based on the ...

Tonsillectomy remains a frequently performed operation in developed countries ensuring that tonsils are the most readily available source of human lymphoid tissue and an easily accessible source of dendritic cells (DC). Tonsil lymphoid tissue also provides a source of the different ...

In the human skin, various types of antigen-presenting cells (APC) are present. In the epidermis, they are identified ultrastructurally as Langerhans cells (LC) by the presence of Birbeck granules. LC are considered to belong to the family of dendritic cells (DC) that are important for the initi ...

The skin acts as a mechanical, physicochemical, and immunological control and defense system. The efficient operation of the skin immune system involves cytokine production and adhesion molecule expression by both infiltrating and resident cutaneous cells, and thus depends upon a s ...

Cells with the morphology of veiled cells were first described in the human intestinal lamina propria in tissue obtained from patients suffering from inflammatory bowel diseases (1,2). These cells were found in greater numbers in inflamed bowel than in normal controls. The isolation of den ...

In the lung several cell types are capable of presenting antigen to T cells. The dendritic cells (DC) are the most potent antigen-presenting cell. DC form a rare cell population in the lung and early studies were hampered because scarce cell populations are seldom easy to isolate. Besides, this cell is p ...

In rheumatoid arthitis patients, three compartments need to be considered: peripheral blood (PB), synovial fluid (SF), and synovial tissue (ST). Dendritic cells (DC) characterized from each compartment have different properties. The methods given are based on cell sorting for isolat ...

Dendritic cells (DC) form a complex network of cells that are distributed throughout the body and whose primary function is the stimulation of antigen-specific immune responses (1). DC are derived from CD34+ progenitor cells located in the bone marrow (2) and undergo a complex process of matura ...

The earliest lymphoid precursor in the adult mouse thymus, the “low CD4 precursor,” was found to be able to produce T cells, B cells, NK cells, and dendritic cells (DC) upon adoptive transfer (1-3). This precursor population represents only 0.03%-0.05% of total thymocytes and expresses low levels of CD4 ...

It has long been known that tissue dendritic cells (DC) are bone-marrow-derived (1,2). However, early attempts to generate DC from the bone marrow gave only low numbers, presumably because maturation and survival signals required in vivo could not be provided in vitro (3,4). The availability of r ...

Dendritic cells (DC) are the most potent antigen-presenting cells (APC) of the immune system. Derived from stem cells in the bone marrow, DC migrate to the tissues where they become “sentinels” of the immune system. DC possess the unique ability to initiate primary T-cell responses and can also stim ...

Dendritic cells (DC) are now recognized as major players in the control of immune responses (1), since they direct both the quality and the extent of the adaptative response. Thus, DC represent a very appropriate means for the manipulation of harmful or protective immunity (2-4).

The dendritic cell (DC) lineage is comprised of bone marrow-derived cells that are present in small numbers in nonlymphoid as well as lymphoid tissues (1). Nonlymphoid DC, such as epidermal Langerhans cells (LC), display an immature phenotype, i.e., they are capable of acquiring and processing ...

Considerable progress in the generation of dendritic cells (DC) from mouse and human precursors has recently been accomplished. Consequently, culture systems are now available for the in vitro generation of large numbers of DC.

Dendritic cells (DC) are professional antigen presenting cells characterized morphologically by the extension of numerous dendrites, phenotypically by the expression of relatively large amounts of MHC class II molecules and costimulatory molecules, and functionally by th ...

Dendritic cells (DC) are “professional” antigen presenting cells (APC) that are pivotal for the initiation of T-cell-dependent immune responses (1). DC are widely distributed cells in the body, but are scarce and thus difficult to purify. Methods have become available to grow DC in culture, fac ...

Dendritic cells (DC) play a pivotal role in the function of the immune system, for they are the primary antigen-presenting cells (APC) in the activation of naive T-lymphocyte responses (1). Recent studies have uncovered complexity in the DC lineage with several subsets, functions, and matura ...

Dendritic cells (DC) are a family of bone-marrow-derived professional antigen presenting cells (APC) with sparse, but wide, tissue distribution (1). They are classified primarily based on their localization: as interdigitating reticulum cells when present in lymphoid organs, as ve ...

Many of the interdigitating dendritic cells (DC) that reside in lymph nodes arise from the migration of tissue interstitial DC such as Langerhans cells in the skin (1). Although this migration appears to be stimulated by cytokines (2), relatively little is known of the mechanisms underlying the ...

Chemotaxis is defined as the directional locomotion of cells sensing a gradient of the stimulus. Some cell types, such as monocytes and neutrophils, can be considered as “professional migrants” and for many years the study of chemotaxis has been applied to these cells. However, other cell types i ...