抗体抗原实验

Chromosomal DNA Extraction from Gram-positive BacteriaThis procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria we've tried.Pellet cells from 10 ...

RNA Isolation Protocol(Revised 5-15-2003) Stabilize RNAStart with 15 ml E. coli Culture containing 7.undefined 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagent (Qiage ...

Isolation of genomic DNA from bacteriaNote: This procedure does not work well with Gram + cocci. Transfer 1.5 mL overnight culture to a 1.5 mL microfuge tube centrifuge for 30 sec decant supernatant.R ...

Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM (Modified from Experimental Techniques in Bacterial Genetics Jones and Bartlet 1990)Materials: TE buffer10% (w/v) sodium dodecyl ...

Isolation and Quantification of Genomic DNA from Mycobacterium tuberculosisThe following protocol describes a "DNA mini-prep" procedure (Part A) for the isolation of chromosomal DNA from Mycobacterium ...

ISOLATION OF RNA FROM BACTEROIDS3 g nodules (fresh or frozen in liquid N2) were ground to a powder in mortar and pestle with liquid N2. To the powder was added ice cold 0.5 M mannitol (or sucrose) 0.0 ...

Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels DNA Preparation E. coli chromosomal DNA is prepared following the method of Heath et al. ( J. Bacteriol. 174 1992). Cells are embed ...

Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired). The following day set up (use polypropylene tubes for ...

Some basic Neurospora methods compiled at Stanford Vogel's Minimal Medium D. D. Perkins - Hints for the care feeding and breeding of Neurospora Strain preservation at the FGSC Toxic concentrations of ...

Hints and precautions for the care feeding and breeding of NeurosporaSome of these notes describe what may be established routines in older laboratories but are either unpublished or published in inac ...

Assessment of Magnaporthe grisea mating type by spore PCRJin-Rong Xu and John E. Hamer - Department of Biological Sciences Purdue University West Lafayette IN 47907Isolation of DNA from filamentous fu ...

Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction T. Freedman and P. J. Pukkila - Department of Biology University of North Car ...

Transformation of Magnaporthe grisea to phosphinothricin resistance using the bar gene from Streptomyces hygroscopicusHei Leung Pat Loomis and Martin L. Pall(1) - Department of Plant Pathology (1)Depa ...

Fungal Genomic DNA ExtractionAuthor: Dr. Eric W. BoehmSource: Contributed by Dr. Eric W. BoehmAbstract: This procedure does not require phenol extraction. The DNA is pure enough for restriction digest ...

A safe method of extracting DNA from Coccidioides immitisAustin Burt(1) Deidre A. Carter(2) Gina L. Koenig(2) Thomas J. White(2) and John W. Taylor(1) (1)Department of Plant Biology University of Cali ...

A simple rapid procedure for the isolation of DNA for PCR from Gibberella fujikuroi (Fusarium section Liseola)N.M. DuTeau and J.F. Leslie - Department of Plant Pathology Throckmorton Hall Kansas State ...

Fungal DNA IsolationSaghai-Maroof MA Soliman KM Jorgensen RA & Allard RW (1984) PNAS 81:8014-8018DNA successfully isolated from fungal species of Cochliobolus Aternaria and Fusarium. The key elements ...

Small scale DNA preps for Neurospora crassa. J. Irelan V. Miao and E.U. Selker - Institute of Molecular Biology University of Oregon Eugene OR 97403-1229Molecular biology experiments often require pre ...

An Ultra-fast method of DNA extraction from NeurosporaEdward B. Cambareri and John A. Kinsey - Department of Microbiology Molecular Genetics and Immunology University of Kansas Medical Center Kansas C ...

A novel method of growing fungi for DNA extractionKeith A. Seifert Centre for Land and Biological Resources Research - Agriculture Canada Research Branch Ottawa Ontario K1A 0C6 CanadaPreparation of fu ...