抗体抗原实验

Double immunofluorescence staining for BCL-6 and else.Any type of tissue is suitable for this technique as long as the antigenicity for your antigen(s) is preserved. Use cytocentrifuged cells or froz ...

FLUORESCENT STAINING OF CELLSFluorescent Phalloidin StainingMaterials1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large ...

Fluorochrome Absorption and Emission SpectraHere we present the absorption and emission spectra of the fluorochromes BD Biosciences Pharmingen conjugates to monoclonal antibodies and other proteins. F ...

Anti-HA ImmunocytologyThe multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO Richmond California) ...

Yeast IF with Methanol/Acetone DehydrationDavid AmbergNote this protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual.This is the protocol to use with t ...

Making Boiled Donkey (or whatever) Serum (BDS) for Blocking You want a final working concentration of 5% Serum. Order the serum from the same company and organism from which you buy secondary antibod ...

Preparing a cell block from cell lines / cell suspensions.Cell lines or purified cell suspensions are a valuable tool to provide known positive controls provide homogeneous tissue in unlimited amount ...

Staining for Surface Receptors contributed by Patricia TsaoThis protocols allows the use of fluorescence microscopy to visualize epitope-tagged receptors expressed by stable adherent cell lines. In co ...

Staining for total receptors contributed by Patricia TsaoThis protocol will visualize both internalized receptors that were once on the plasma membrane and receptors in the biosynthetic pathway. This ...

Figure 12.4 Typical bacterial shapes Materials Colonies of bacteria from Exercise 12.2ToothpicksCrystal violetGram's iodine95% ethanolSafraninMicroscopes with oil immersion Procedure Before staining ...

Gram-staining ProcedureGram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should be mounted and fixed on a s ...

Analysis of total E. coli protein by SDS PAGEHahn Lablast modified 9/30/981. In microfuge tubes spin down 0.1 ml of uninduced cells grown to near saturation or 0.15 ml of IPTG induced cells. Remove YT ...

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEINIndirect MethodMaterials1. Gc-globulin (vitamin-D binding protein; Calbiochem 345802) prepare 2 mg/ml stock in PBS solution A clarify 25000 ...

Vital Staining of Chromosomes with Hoechst 33258Materials1. Hoechst 33258 10 mg/ml stock in DMSO. 2. Culture medium. 3. 15 ml sterile conical tube. Procedure1. In a 15 ml conical tube add 1 volume Hoe ...

OUTLINEThe erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts.PRO ...

OUTLINEClodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also tak ...

Enrichment of PBMCs with monocytesContributed by Dr.A.GratchevThis protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this proto ...

OUTLINEThis modification of peritoneal macrophage preparation is designed for a subsequent thrombophagocytosis test. PROTOCOLSacrifice a mouse (use dry ice CO2) Sink a mouse in the 5% water solution ...

Phagocytosis Fluorescent labeling of yeastResuspend 5 grams of yeast in 50 ml PBS in 100 ml flask Put flask for 30 minutes in boiling water bath while stirring. Wash 5 x with PBS and 2 x in PB. Adjust ...

Purification of human mononuclear cells and neutrophilsby Chang-Duk Jun 01/19/2000 PurposeMaterials10ml 6% dextran + 7ml citrate/citric acid Dextran: T500 -- 6g+100ml PBS Citrate solution: 25g Na Citr ...