实验仪器使用技术

COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions-InterferencesAbsorbance 280nm0.1-1 OD280nm/mlAbsorbance of aroma ...

1.1. Can the NCIS Kit be used on adults?The NCIS kit is specifically packaged for five separate tissue dissociations each containing up to twelve neonatal rat hearts. The NCIS protocol is available on ...

Cell Isolation Techniques Methods and MaterialsWorking With EnzymesAll of the enzymes Worthington offers for tissue dissociation applications are available as lyophilized powders for convenience vers ...

Cell Isolation Techniques Methods and MaterialsWorking With EnzymesAll of the enzymes Worthington offers for tissue dissociation applications are available as lyophilized powders for convenience vers ...

Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap tubes. 2. Add 4 ml water centrifuge @ 2000 rpm in b ...

Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap tubes. 2. Add 4 ml water centrifuge @ 2000 rpm in b ...

Lowry � Protein Determination(From Protein Protocols on CD-ROM Humana Press 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Waterborg and Harry R. Matthews) (Lowry O. H. Rosebrou ...

Protein concentration of Laemmli gel samplesTo 10 µl boiled lysate (in Laemmli sample buffer) add 40µl water + 50µl 50% TCA. Ppt. 10 min. on ice. Spin and wash with 10% TCA. Resuspend pellet in 100 µl ...

Protein Assay (Spectrophotometer)Use BSA (bovine serum albumin) 1mg/ml stock solution (1ml Eppendorf tubes) for standard curve. Place 0 2 5 10 15 20µg (=µl) BSA into plastic cuvette. Use 2-5µl of samp ...

Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibod ...

Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibod ...

UV Absorbance (280 nm) � Protein Determination Simple and quick method to accurately quantitate total protein in purified material or approximately quantitate total protein in crude lysates or partia ...

Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the donor. Disproportionate numbers of white cells (l ...

Senescent-associated b-Gal AssayWash cells with PBS Fix cells in 0.2% glutaraldehyde for 5’ dilute this in PBS Wash cell with PBS Stain with X-gal solution (ingredients listed below) for 6 to 24 hours ...

DNA Molecular Weight MarkersLambda DNA Hind III DigestFragmentBase Pairs123310294153655744361523226202775648125Lambda DNA EcoR I DigestFragmentBase Pairs1212262742135804456435487863530Lambda DNA Hind ...

REBASE The Restriction Enzyme Database The Restriction Enzyme data BASE A collection of information about restriction enzymes and related proteins. It contains published and unpublished references rec ...

Announcement Codon Usage Database is an extended WWW version of CUTG (Codon Usage Tabulated from GenBank). The frequency of codon use in each organism is made searchable through this World Wide Web si ...

Molecular Weight Markerl HindIII Digest Marker Solution Digest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl) Add 50 µl 10X Tracking Dye Bring volume of the digested DNA up to 500 µl with H2O (410 µl if the ...

Alphabetic List of Commercial Isoschizomers and Corresponding Fermentas Restriction EndonucleasesInformation on commercially available restriction endonucleases is from:Roberts R.J. and Macelis D. Nuc ...

Asymmetric sequences are indicated by *.Single letter code:R = G or A;Y = C or T;W = A or T;M = A or C;K = G or T;S = C or G;H = A C or T;V = A C or G;B = C G or T;D = A G or T;N = G A T or C.Re ...