实验仪器使用技术

Sections 5-10 micron unfixed or cold formol calcium/gum sucrose fixed Cryostat sections.Stock solutionsA) Pararosanilin-HCL StockPararosanilin 1gDistilled water 20mlHCl Conc 5mlThe pararosanilin is di ...

Procedure 1. After sperm preparation adjust sperm concentration as high as possible (5-10 x 106/ml). 2. Prepare a smear of sperm suspention onto a slide and allow it to dry. 3. Place slides in 0.1% (w ...

Procedure 1. After sperm preparation adjust sperm concentration as high as possible (5-10 x 106/ml). 2. Prepare a smear of sperm suspention onto a slide and allow it to dry. 3. Place slides in 0.1% (w ...

SECTIONSUnfixed cryostat or cold Formol calcuim/gum sucrose fixed on muscle biopsy sections.SOLUTIONSa. 0.1M Glycine buffer.0.75g glycine + 0.585g NaC1 made up to 100ml with distilled water.b. O.1M gl ...

1. Bring sections to water 2. Stain in Celestine blue 3 mins 3. Wash in water 4. Stain in Carazzi Haemalum 3 minutes 5. Wash in water 6. Blue in Scotts tap water 7. Wash in water 8. Rinse in 95% alcoh ...

SECTIONSUnfixed cryostat or cold Formol calcuim/gum sucrose fixed on muscle biopsy sections.SOLUTIONSa. 0.1M Glycine buffer.0.75g glycine + 0.585g NaC1 made up to 100ml with distilled water.b. O.1M gl ...

1. Bring sections to water 2. Stain in Celestine blue 3 mins 3. Wash in water 4. Stain in Carazzi Haemalum 3 minutes 5. Wash in water 6. Blue in Scotts tap water 7. Wash in water 8. Rinse in 95% alcoh ...

1. Bring sections to water 2. Acidified potassium permanganate 2 minutes 3. Wash in water 4. 1% Oxalic acid 1 minute 5. Wash in water 6. Wash in 70% alcohol (optional) 7. Stain in Weigert's Resorcin F ...

1. Bring sections to water. 2. Acidified potassium permanganate 2 minutes 3. Wash in water 4. 1% Oxalic acid 1 minute 5 Wash in water 6. Wash in 70% alcohol (optional) 7. Stain in Weigerts Resorcin Fu ...

SECTIONSFresh Frozen (jejunal Biopsy) or cold Formal calcium/Gum Sucrose fixed Cryostat 5-7micron thick.SOLUTIONSa. 0.2M Tris HCL buffer pH 8.30.2M Tris (2.42g/100) 25ml 125ml )0.1N HCL (0.85/110ml) 2 ...

I. Purpose:A. To locate nucleolar organizer regions on chromosomes. Useful for studies of chromosomes with double satellites chromosome polymorphisms and structural abnormalities involving satellite r ...

Mucin stainsThere are a variety of mucin stains all attempting to demonstrate one or more types of mucopolysaccharide substances in tissues. The types of mucopolysaccharides are as follows:Neutral - T ...

SECTIONS4µm paraffin wax sectionsSOLUTIONS1. STOCK ALCOHOLIC SALT SOLUTION--Saturated sodium chloride in 80% alcohol2. WORKING SOLUTION--Stock solution (1) 50cm3 --1% Sodium hydroxide 0.5cm3 --Mix fil ...

SECTIONS8micron paraffin wax sections.SOLUTIONS1. Silver A - 20% silver nitrate2. Reducer (Make fresh) Formaldehyde 50mlAbsolute Alcohol 200ml Distilled water 750ml 3. Silver B20% Silver nitrate 20ml ...

SECTIONS4micron Paraffin wax sectionsSOLUTIONSAlcian Blue pH 3.1Alcian Blue 1g0.5% Acetic Acid 100mlAlcian Blue pH 2.5Alcian Blue 1g3% Acetic Acid 100mlAlcian Blue pH 1.0Alcian Blue 1g0.1M Hydrochlori ...

SECTIONS8µm paraffin wax sectionsSOLUTIONSSTAINING SOLUTIONCresyl Fast Violet-1g Distilled water-100cm3 Acetic acid-0.25cm3METHODTake sections to water Stain in cresyl fast violet solution for 20-30 m ...

SECTIONS4micron paraffin wax sectionsSOLUTIONS1. Alcian Blue pH 2.5Alcian Blue 1g3% Acetic Acid 100ml2. 1% Periodic Acid 3. Schiffs ReagentMETHODTake sections to waterPlace in Alcian Blue for 5 minute ...

Procedure:Incubate sections in the incubating medium at room temperature for 1 - 3 hours. Two hours is sufficient in most cases. Wash in distilled water for 2 minutes. Counterstain with Nuclear Fast R ...

Incubate sections in the incubating medium at 37°C for 5 - 12 hours. Long incubation periods are needed to get significantly visible reaction product. Wash in distilled water for 2 minutes. Countersta ...

Embedding in plastic provides many advantages to the histotechnologist. Thinner sections can be made providing improved detail. Better support is given to cellular components offering improved morphol ...