BIELSCHOWSKY METHOD

 

SECTIONS
8micron paraffin wax sections.
 

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SOLUTIONS

1. Silver A - 20% silver nitrate
 

2. Reducer (Make fresh)

• Formaldehyde 50ml
• Absolute Alcohol 200ml
• Distilled water 750ml

3. Silver B

• 20% Silver nitrate 20ml
• 95% Alcohol 20ml
• Add ammonia until precipitate formed is redissolved
• Add 10 more drops of ammonia

4. 5% Sodium thiosulphate

5. 0.1% Gold chloride

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METHOD

 

1. Take sections to distilled water
2. Place in solution A for 2-3 hours
3. Wash in reducer for 2-5 minutes
4. Wash in 10% alcohol for 2 minutes
5. Place in solution B for 1-2 minutes
6. Wash in a constant flow of reducer and leave for 3-5 minutes
7. Wash in distilled water
8. Tone 0.1% gold chloride for 2 minutes
9. Wash in distilled water
10. Fix in 5% sodium thiosulphate for 2 minutes
11. Wash in tap water
12. Dehydrate, clear . Mount sections in DPX

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RESULTS
Neurites around senile plaques, neurofibrillary tangles - Black.