实验仪器使用技术

Although patch clamp set-ups range from a simple rig to the mostelaborate patch clamp arrangements in which a large number of variablesare carefully controlled, there is a basic set of conditions that must be metin all cases for patch clamping to work. These conditions will be discussedin this Ch ...

startup• Turn on the scope (switch on base, toward back, on the right).• Turn on the mercury lamp for the scope (small white box to the left of the scope).• Turn on the green laser (far right) by pushing the red button, turning the key to the right, and turning up the power to about 10 o’clock on the dial.• Turn on the red laser (next to g ...

1. Cleaning coverslips (use gloves)Add 1 bottle of Chromerge, 1/5 at a time, into 2.5 L bottle of Sulfuric Acid. Invert the bottle to mix after each addition.Load coverslips into glass carriers in every other slot at a slant (see fig. 1).Pour enough chromic acid solution into tray to cover the tops of the covers ...

Make-up 300 ml of 2 parts Nitric Acid - 1 part HCl in a glass beaker in the hood. Solution will turn orange-red. Place #1.5 coverslips into solution a few at a time. Let sit for about 2 hrs. with occasional swirl. Decant acid into waste bottle carefully. Wash extensively in running water until pH of water is back up to 5.5 -6.0. S ...

The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool in biology. Yet, many students and teachers are unaware of the full range of features that are available in light microscopes. Since the cost of an ins ...

The use of radioactive tracers in cell research is an effective and safe means of monitoring molecular interactions. There is simply no other technique which allows the precision and specificity of radioactive tracers. Radiation is to be taken seriously. At a minimum, its misuse can lead to incr ...

Each time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance, this could result in objects in the preparation being undetected. There are many microscope set up procedu ...

Materials Needed20ml glass scintillation vial Small stir bar Foil Glycerol 1X PBS Pipets * P-phenylenediamine ( EMD Chemicals Inc. Cat# PX0730) Carbonate-Bicarbonate Buffer (see below) Wrap a glass scintillation vial with foil and drop in a small stir bar. (PPD is light-sensitive.) With a 10 ml p ...

Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techniques. Sterile technique refers to procedures by which cultures may be manipulated without infecting the worker or contaminati ...

Hahn LabCheck manuals for more detailed information.Please Note: We have been having problems with the seal and bearing assembly at the bottom of the unit which allows the shaft to rotate inside the growth chamber. It is important to always have seal steam pressure (10-15 psi) when stirring the media, ...

PEAS Labeling Gcn4-cysJames Fishburn, Hahn Lab March 2006Notes10 mCi 125I = 4.6 nmol (see spec. sheet for calculation)4.6 nmol Gcn4(1-118,209-281) = 103 microgram (M.W. 22,400)PEAS has low solubility in aqueous solutionMinimize the time Gcn4 is without DTT by preparing the protein just befo ...

Ampicillin (50mg/ml)Ampicillin Dissolve Ampicillin (Na salt from Sigma, A-9518) in H20. Store 1 ml aliquots at 20oC in common freezer for stock solutions 上一篇：PREPARATION OF PROTEIN A SEPHAROSE CL 4B BEADS 下一篇：ALPHA FACTOR ARREST AND RELEASE

Blotting with BSA Blot in 5% mile/ 1X PBST (or 1X TBST) --60'/Rt Wash 2X for 5' in 1XPBST 10Ab in 1% BSA/1X PBST --60'/RtWash PBST 15', then 3x for 5' each in 1X PBST20Ab in 1% BSA/1X PBST --60'/Rt (aHRP)--Wash 15' with 1X PBST then 3x with PBST for 5' @Chemiluminescence: Mix 1:1 NEN reagents. Add to Mb --1'/shaking. Blot excess Put between s ...

GLASS BEADS0.45 - 0.5mm beads (Sigma)Clean by soaking in nitric acid --stir bar, O/N.****Note, dispose of acid in appropriate flask.Wash w/ lots distilled water (til pH to that of water, allow to soak ~2H between changes to ensure clean)Dry before use (bake in oven)Microbeads: Cataphote Inc. 1-800-221- ...

Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20oC in common freezer for stock solutions 上一篇：ALPHA FACTOR ARREST AND RELEASE 下一篇：GLASS BEADS

Alpha Factor Arrest and ReleaseAlpha factor stock is 10-3 M in 0.1N HCl (i.e. resuspend 5 mg alpha factor in 2.97 ml 0.1N HCl). To arrest BAR1 strains, the pheromone should be diluted to a final concentration of approximately 3 x 10�5 M, while bar1 strains require much lower pheromone concentrations (10-8M). To ...

RNAase Dissolve in 50 mM KAC pH 5.5 Heat to 100 oC for 15 minutes and cool slowly to room temperature Store at -20. (common freezer for stock solutions) 上一篇：GLASS BEADS 下一篇：Salmon Sperm DNA (10mg/ml)

SCE 0.5 M Sorbital 0.05 M Na Citrate 0.005 M EDTA pH to 5.8 上一篇：Salmon Sperm DNA (10mg/ml) 下一篇：SPECTROPHOTOMETER INSTRUCTIONS

2nd Floor Spectrophotometer w/ monitorTurn on power switch. Bring up Menu (hit Mode button?) at top of screen; it asks if you want to make changes? Y or N. Hit Yes. Hit the number 1. (for changing wavelength) Hit Enter. type in wavelength ex. 260 Hit Enter. Now you're back at the main Menu which asks if you want to make changes ? Y or ...

POSI BLOT SOLUTIONS2X Posi blot denaturing sol'n2 Liters80g NaOH sodium hydroxide (pellets)350.6g NaCl sodium chloridebring up to 2 liters with H2Ostir until dissolvedaliqot into two 1-liter bottlesdo not autoclave2X Posi blot neutralization sol'n2 Liters484.4g Tris basebr ...