遗传学实验技术

Conventionally, expression plasmids in Escherichia coli have generally been constructed using ligation reaction-assisted cloning followed by the generation of inserts. In such cases, the insert was generated by polymerase chain reaction (PCR), digestion using restriction e ...

Nucleopolyhedrovirus, a baculovirus, generates many intranuclear polyhedra in lepidopterous insects. The replacement of the polyhedra gene with a target gene, under a potent polyhedrin promoter, is widely used to express recombinant proteins. In this chapter, we describe the app ...

The Mouse P19 cell line was derived from an embryonal carcinoma. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells. Here, we describe an miRNA microarray method to monitor the miRNA expression profiles during the course of P19 cells neuronal differentia ...

MicroRNAs (miRNAs) are a class of ∼22-nucleotide endogenous noncoding RNAs which regulate target gene expression via repressing translation or promoting mRNAs degradation. Any individual mammalian miRNA often has more than a hundred predicted mRNA targets and that close to one thi ...

In the recent past, microRNAs (miRNAs) have gained significant attention as potent regulators of gene expression. These small noncoding RNA molecules are currently of major interest when investigating regulatory circuits of the cell. After identification of potential miRNA–ta ...

MicroRNAs (miRNAs) are single-strand nonprotein coding RAN with 18 to 25-nucleotides long. With complementary sequence to target messenger RNA (mRNA), miRNA regulates mRNA degradation and protein translation. miRNAs have been identified in various organisms ranging from virus ...

In the recent past, intragenic microRNAs (miRNAs) have gained significant attention. Due to the unique linkage to their host gene’s transcription, these miRNAs offer more information than intergenic miRNAs as they associate with some of their hosts’ properties. However, genome wide ana ...

MicroRNA (miRNA) and retroelements may be a master of regulator in our life, which are evolutionally involved in the origin of species. To support the Darwinism from the aspect of molecular evolution process, it has tremendously been interested in the molecular information of naive RNA. The RNA ...

Second-generation high-throughput sequencing is a robust and inexpensive methodology that is becoming an increasingly common technique for the study of microRNA (miRNA) expression levels in the central nervous system. This method allows for the identification of both known and no ...

miRNAs are a large subgroup of noncoding regulatory RNAs, which vary in length within the 20–25 nt range and show substantial length diversity and heterogeneity. To analyze the latter phenomenon, we recently developed high-resolution northern blotting and employed this method to inve ...

Prostate cancer (PC) is the most prevalent strain of cancer in men, but it is often slow-acting or undetected. Common diagnostic tools for PC include prostate biopsy and consequent analysis by the Gleason scoring of the tissue samples, as well as tests for the presence and levels of prostate-specif ...

MicroRNAs (miRNAs) are small noncoding RNA molecules ∼22 nucleotides in length that can post-transcriptionally repress gene expression. MiRNAs bind to their target messenger RNAs (mRNAs), leading to mRNA degradation or suppression of translation. miRNAs have recently been shown ...

MicroRNAs (miRNA) are short, single-stranded, noncoding RNAs that regulate a number of cellular processes through their capability to silence the expression of target proteins. Using miRNAs to target and reduce the expression of tyrosinase, the key enzyme involved in the synthesis of sk ...

MicroRNAs (miRNAs) in human saliva have recently become an emerging field in saliva research for �diagnostics applications and its potential role in biological implications. miRNAs are short noncoding RNA molecules that play important roles in regulating a variety of cellular proc ...

Today’s researchers generating induced pluripotent stem cells (iPS cells or iPSCs) usually consider the pluripotency first, then, the potential tumorigenicity. Oncogenic factors such as c-Myc and Klf4 were frequently used to boost the survival and proliferative rates of iPSCs, cre ...

MicroRNAs (miRNAs) are 21–23-nucleotide-long small noncoding RNAs, function as mediators in gene silencing, and play essential roles in gene regulation in development, differentiation, and proliferation. Hundreds of miRNAs have been found, and tissue-specific or organ-speci ...

MicroRNAs (miRNAs), widely distributed, small regulatory RNA genes, target both messenger RNA (mRNA) degradation and suppression of protein translation based on sequence complementarity between the miRNA and its targeted mRNA. Different names have been used to describe various t ...

Clinical implications of induced pluripotent stem (iPS) cell technology are enormous for personalized medicine. However, extensive use of viral approach for ectopic expression of reprogramming factors is a major hurdle in realization of its true potential. Non-viral methods for m ...

Vector-based methods for manipulating microRNA (miRNA) activity in vivo and in vitro face a number of disadvantages regarding biosafety, workload, and potential for therapeutic use in patients. Use of miRNA mimics and inhibitors provides an alternative for enhancing or inhibiting t ...

RNA interference (RNAi) has emerged as a powerful tool in basic research and therapeutics by silencing the expression of specific target genes. RNAi is triggered by double-stranded small interfering RNAs, which can be processed from naturally or artificially expressed microRNAs (mi ...