生物信息学技术

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Abstract Table of Contents Figures Literature Cited Abstract OrthoMCL is an algorithm for grouping proteins into ortholog groups based on their sequence similarity. OrthoMCL?DB is a public database that allows users to browse and view orth

Abstract Table of Contents Figures Literature Cited Abstract This unit describes how to use the gene?finding programs GeneMark.hmm?E and GeneMark?ES for finding protein?coding genes in the genomic DNA of eukaryotic organisms. These bioinfo

Abstract Table of Contents Figures Literature Cited Supplementary Material Abstract This unit describes how to use several gene?finding programs from the GeneMark line developed for finding protein?

Abstract Table of Contents Figures Literature Cited Abstract Analysis of genomic data requires access to software tools that place the sequence?derived information in the context of biology. The Saccharomyces Genome Database (SGD) integrat

Abstract Table of Contents Figures Literature Cited Abstract Phylogenetic analysis is the study of evolutionary relationships among molecules, phenotypes, and organisms. In the context of protein sequence data, phylogenetic analysis is one

Abstract Table of Contents Figures Literature Cited Abstract Protein engineering is altering the structure of a protein to improve or change its properties. This unit summarizes concepts for protein engineering using rational design, direc

Abstract Table of Contents Figures Literature Cited Abstract Recognition and prediction of structural domains in proteins is an important part of structure and function prediction. This unit lists the range of tools available for domain pr

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes experimental and analytical procedures for characterizing the efficiency and fidelity of translesion DNA synthesi

Abstract Table of Contents Figures Literature Cited Abstract The recent plethora of proteomic mass spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We des

Abstract Table of Contents Figures Literature Cited Abstract LabKey Server (formerly CPAS, the Computational Proteomics Analysis System) provides a Web?based platform for mining data from liquid chromatography?tandem mass spectrometry (LC?

Abstract Table of Contents Figures Literature Cited Abstract miRNAs comprise an abundant class of small non?coding RNAs that play important roles in a wide range of biological processes by post?transcriptional regulation of a large fractio

Abstract Table of Contents Literature Cited Abstract Next?generation sequencing (NGS) packs the sequencing throughput of a 2000's?era genome center into a single affordable machine. However, software developed for conventional sequencing technologies is often inadequate ..

Abstract Table of Contents Figures Literature Cited Abstract Molecular interactions are key processes that drive the functions of molecules. Large data sets of protein interaction data are being assembled that will help analyze structural

Abstract Table of Contents Figures Literature Cited Abstract Many patients suffering from developmental disorders have submicroscopic deletions or duplications affecting the copy number of dosage?sensitive genes or disrupting normal gene e

Abstract Table of Contents Materials Figures Literature Cited Abstract The recent success of genome?wide association studies has generated a trove of biologically significant variants implicated in human

Abstract Table of Contents Materials Figures Literature Cited Abstract Reactive nitrogen species (RNS) and reactive oxygen species (ROS) are derived as a result of inflammation and oxidative stress and ca

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the isolation of activated Fc receptor complexes from RAW 264.7 macrophages using live?cell affinity receptor chr

Abstract Table of Contents Materials Figures Literature Cited Abstract DNA origami is an emerging technology for designing defined two? and three?dimensional (2D and 3D) DNA nanostructures. Here, we repor

Abstract Table of Contents Figures Literature Cited Abstract MAVEN is an open?source software program for interactive processing of LC?MS?based metabolomics data. MAVEN enables rapid and reliable metabolite quantitation from multiple react