生物信息学技术

Abstract Table of Contents Materials Figures Literature Cited Abstract This method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti?sense primers directed ag

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit presents a method for the amplification of poly(A)+ mRNA extracted from the cytoplasm of a single cell. After cDNA is synthes

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit contains protocols for expression cloning in mammalian cells. Either calcium phosphate? or liposome?mediated transfection o

Abstract Table of Contents Materials Literature Cited Abstract Expression cloning requires a representative cDNA or genomic DNA library and a host organism in which the cloned genes can be transcribed and/or translated. It likewise require

Abstract Table of Contents Materials Figures Literature Cited Abstract Of the many proteins that are known to be involved in neuronal signaling, one family of gene products, collectively referred to as th

Abstract Table of Contents Materials Literature Cited Abstract The principle of cloning utilizes hybridization of single?stranded DNA probes to denatured DNA fixed on membranes to detect the identical DNA molecules present in a large popul

Abstract Table of Contents Figures Literature Cited Abstract Synthetic nucleic acids are produced routinely for a wide variety of applications, including biological and chemical research, and diagnostic or therapeutic applications. To ensu

Abstract Table of Contents Figures Literature Cited Abstract Database sequence similarity searching is carried out thousands of times each day by researchers worldwide and has become a very valuable tool. Over the years, a number of algori

Abstract Table of Contents Figures Literature Cited Abstract This unit outlines a variety of methods by which DNA sequences can be manipulated by computers. Procedures for entering sequence data into the computer and assembling raw sequenc

Abstract Table of Contents Figures Literature Cited Abstract This unit describes the NCBI's Entrez database browser. Entrez integrates DNA and protein sequence data, threedimensional structures, and taxonomic information with its associate

Abstract Table of Contents Figures Literature Cited Abstract With the explosion of sequence and structural information available to researchers, the field of bioinformatics is playing an increasingly large role in the study of fundamental

Abstract Table of Contents Figures Literature Cited Abstract This brief appendix serves as a guide for the analysis of functional motifs in proteins. Several database search engines that can be accessed via the World Wide Web are described

Abstract Table of Contents Materials Figures Literature Cited Abstract Specific sequences in RNA preparations can be detected by blotting and hybridization analysis using techniques very similar to those

Abstract Table of Contents Figures Literature Cited Abstract This unit introduces readers to some of the more commonly used techniques for gene identification. The author discusses the general problem behind accurately predicting genes in

Abstract Table of Contents Figures Literature Cited Abstract DNA microarrays or chips can be used to simultaneously monitor the expression levels and/or genotypes of thousands of genes. The application of these techniques heralds a new era

Abstract Table of Contents Materials Figures Literature Cited Abstract The ability to construct comprehensive gene expression profiles comprising hundreds to thousands of genes whose RNA levels are monito

Abstract Table of Contents Figures Literature Cited Abstract Nucleic acid array technology refers to the use and fabrication of arrays containing thousands of nucleic acid samples bound to solid substrates such as glass microscope slides o

Abstract Table of Contents Materials Figures Literature Cited Abstract cDNA Library Construction from Single Cells (Phillip R. Kramer, National Institute of Neuronal Disorders and Stroke, NIH, Bethesda, M

Abstract Table of Contents Literature Cited Abstract The International Union of Pure and Applied Chemistry (IUPAC) and the International Union of Biochemistry and Molecular Biology (IUBMB) have established standards for representing nucleic acids and amino acids with single

Abstract Table of Contents Figures Literature Cited Abstract TreeView provides a simple way to view the phylogenetic trees produced by a range of programs, such as PAUundefined, PHYLIP, TREE?PUZZLE, and ClustalX. While some phylogenetic programs