生物信息学技术

Abstract Table of Contents Figures Literature Cited Abstract Receptors, located on both the cell surface and within the cell, are the molecular targets through which drugs produce their beneficial effects in various disease states. Recepto

Abstract Table of Contents Materials Figures Literature Cited Abstract Peptide conjugation of oligonucleotides and their analogs is being studied widely towards improving the delivery of oligonucleotides

Abstract Table of Contents Materials Figures Literature Cited Abstract Chromosome conformation capture (3C) is one of the only techniques that allows for analysis of an intermediate level of chromosome st

Abstract Table of Contents Figures Literature Cited Abstract Radioligand binding has been used for many years to identify new binding sites, characterize receptors, and identify novel ligands. Although various techniques have been develope

Abstract Table of Contents Materials Figures Literature Cited Abstract A general approach to phosphoramidites tethered with single and multiple linkers through the use of methoxyoxalamido (MOX) chemistry

Abstract Table of Contents Materials Figures Literature Cited Abstract A concept that has arisen over the last decade is that proteins can, in general, be covalently modified by poly

Abstract Table of Contents Materials Figures Literature Cited Abstract The optical phenomenon of surface plasmon resonance (SPR) used by Biacore systems enables the detection and measurement of protein?pr

Abstract Table of Contents Literature Cited Abstract Surface plasmon resonance (SPR) allows for the investigation of the functional nature of binding interactions and provides detailed kinetic information across a wide range of molecular weights, including small molecules,

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes how fluorescence quenching methods can be used to determine binding constants for phospholipids binding to intrin

Abstract Table of Contents Figures Literature Cited Abstract Fluorescence resonance energy transfer (FRET) refers to the nonradiative transfer of energy from one fluorescent molecule (the donor) to another fluorescent molecule (the accepto

Abstract Table of Contents Materials Figures Literature Cited Abstract Pulse proteolysis exploits the difference in proteolytic susceptibility between folded and unfolded proteins for facile but quantitat

Abstract Table of Contents Figures Literature Cited Abstract Circular dichroism (CD) is a powerful technique for studying the structures of proteins in solution, as well as structural changes that may occur when proteins bind to ligands. C

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit outlines methods for identifying cyclic peptides that inhibit protein?protein interactions. Proteins of interest are cloned

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes ligand binding assays for the characterization of the benzodiazepine (BZ) site of GABAA receptors in the central ne

Abstract Table of Contents Materials Figures Literature Cited Abstract The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three?dimensional str

Abstract Table of Contents Materials Figures Literature Cited Abstract Chemical crosslinking provides information about protein?protein interactions in the context of intact protein complexes; therefore,

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method for analyzing protein complexes by chemically cross?linking closely positioned amino acids. The cross?li

Abstract Table of Contents Materials Figures Literature Cited Abstract Dopamine receptors are the principal targets of drugs used for the treatment of neuropsychiatric disorders such as schizophrenia and

Abstract Table of Contents Materials Figures Literature Cited Abstract Norepinephrine and epinephrine are important neurotransmitters in both the peripheral and the central nervous systems. The actions of

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes radioligand binding protocols for histamine H1, H2, H3, and H4 receptors. Because these rec