植物学技术

Considering the economic importance of cotton in many developing and developed countries, there is an urgent need to accelerate the application of biotechnological tools to address the problems associated with the production of this crop and to improve the quality of fiber and seed. This re ...

Sunflower (Helianthus annuus L.) is considered one of the recalcitrant species in terms of transformation and regeneration. A routine transformation system of this crop requires competent cell cultures for efficient plant regeneration as well as an effective method for gene delive ...

A protocol for rapid, highly efficient transformation of alfalfa is described. Leaf explants from growth chamber-grown plants of a highly regenerable genotype are surface-sterilized, the margins are removed, and explants are inoculated with Agrobacterium tumefaciens strain L ...

Chickpea is one of the most important leguminous, cool-season, food crops, cultivated prevalently in the Asian Pacific region. In spite of its nutritional importance, its area of cultivation has been low, with virtually no increase. Conventional breeding has resulted in several importa ...

Legumes constitute one of the most important global groups of agricultural species, providing a major source of protein and oil for humans and animals as well as fixing nitrogen and improving the fertility of soils. Gene technology can assist plant improvement efforts in clovers (Trifolium ...

In this chapter we describe a robust method for transformation of peas that has been successfully used in our laboratory since 1992. Using immature pea seed collected from field- or greenhouse-grown plants, we have produced transgenic lines for over 30 genotypes including named pea cultiva ...

Arachis hypogea (peanut, groundnut), an annual oil seed belonging to the Leguminosae family and the Papillionacea subfamily, is a legume native to South America but now grown in diverse environments in six continents between latitudes 40�N and 40�S. Arachis hypogea can grow in a wide range of cli ...

Pigeonpea , also known as redgram, is one of the major grain legume (pulses) crops grown in the semiarid tropics (SAT) extending between 30�N and 30�S; it is the second most important food legume of India. It is cultivated in about 50 countries of Asia, Africa, and the Americas for a variety of uses (food, fodder, f ...

Agrobacterium tumefaciens-mediated transformation of soybeans has been steadily improved since its development in 1988. Soybean transformation is now possible in a range of genotypes from different maturity groups using different explants as sources of regenerable cells, v ...

Genetic modification of plants by the insertion of transgenes can be a powerful experimental approach to answer basic questions about gene product function. This technology can also be used to make improved crop varieties for use in the field. To apply this powerful tool to red clover, an importa ...

Agrobacterium tumefaciens-mediated transformation of soybeans can be accomplished using immature zygotic cotyledons as target tissues providing an alternate explant to embryogenic tissue cultures, proliferating meristems, and cotyledonary nodes. The immature cot ...

Phaseolus beans are among the major legumes for food consumption, especially in Latin America, Africa, and Asia. Tepary bean (Phaseolus acutifolius L. Gray) is one of the five cultivated species of the genus Phaseolus. This chapter describes an Agrobacterium-mediated transformation p ...

A better understanding of the genetic basis underlying the genotype dependence of Brassica oleracea transformation is enabling researchers to distinguish between recalcitrant and successful candidate genotypes for routine transformation. In this chapter we outline an A. tu ...

We describe two novel Agrobacterium tumefaciens-based methods of cucumber transformation. The first involves direct regeneration from leaf microexplants selected on kanamycin-containing medium. The second involves regeneration from a long-term established embryo ...

Eggplant is an economically important vegetable crop in Asia and Africa, and although it is grown in Europe and the United States, it does not account for a significant percentage of agricultural production. It is susceptible to a number of pathogens and insects, with bacterial and fungal wilts be ...

Tomato (Lycopersicum esculentum) is an important fruit crop in the Americas, southern Europe, the Middle East, and India, with increasing production in China, Japan, and Southeast Asia. It is amenable to producing pharmaceuticals, particularly for oral delivery; for many of the same reaso ...

A well-controlled growth environment with plants that are not unduly stressed is essential for Arabidopsis molecular biology research. Even if they do not kill the plants outright, insect pests and microbial pathogens can cause subtle changes in gene expression or plant metabolism that ...

Recent interest in the study of biological phenomena in Brassicaceae members (crucifers) related to the model plant, Arabidopsis thaliana, fuels the need for standard protocols for growth and maintenance of these plant species. Like A. thaliana, many wild crucifers are amenable to growth ...

Grafting provides a simple way to generate chimeric plants with regions of different genotypes, and thus to assess the cell autonomy of gene action. The technique of grafting has been widely used in other species, but in Arabidopsis, its small size makes the process rather more complicated. Howe ...

We have recently described the selection of rapidly dividing Arabidopsis cell suspension cultures MM1 and MM2d that provide a powerful platform for plant cell-cycle research. Here we provide detailed protocols and procedures to achieve high levels of synchronization, either by sta ...