实验动物技术

Cuticle preparationsThis procedure was adapted from one described over the phone (thanks P.G.). We're not sure if it appears elsewhere in published form.-Collect embryos on apple juice-agar plates for ...

GENERAL MAINTENANCE INSTRUCTIONS FOR IMAGINAL DISC CELL LINES This is our lab protocol for growing imaginal disc cells (clone-8) The Milner Lab website has additional protocols for clone-8 cells. Cl ...

This is a firm medium that keeps well and makes a good base for egg collecting. Wrap filled petri dishes and store at 4-- 14oC (they should keep for at least 6 weeks). Substitute molasses for the c ...

Sporulation in Liquid Media1) Grow cells in 25mls of PSP2 (in 250ml flask) supplemented with 25% of the recommended concentration of amino acids until they are about 1x107 cells/ml (~1 day).2) Wash wi ...

YPD FILLED MICROTITER PLATES Uses: 1. Grow individual strains to use for PCR2. Shipping strains 1. Make YPD regular agar (1.5-2%) for sending strains.2. Make 200ml batches = enough for 6 microtiter pl ...

HC Dropout Plate Supplementation TableAmino AcidStockAmount To Spread(per 25 ml plate)URA1 mg/ml875 µlADE1 mg/ml500 µlLEU20 mg/ml100 µlLYS10 mg/ml300 µlMET2 mg/ml250 µlHIS10 mg/ml50 µlTRP10 mg/ml200 µ ...

Fly extract 2 for routine culture (FE2) 1. First collect your flies; tip them from culture bottles into an empty glass bottle and plug it. Put it in the freezer for 45 mins or so until the flies are ...

Production of Conditioned Medium containing Wingless protein from S2 cells- Grow heatshock wingless S2 cells at 25 degrees centigrade in a tissue culture flask to approximately 2.5 x 106 cells/ml.- He ...

Medium Shields and Sang Medium 3 (SS3) modified for low serum. Supplemented before use with 2% heat inactivated FCS 2.5% FE2 12.5IU/100ml insulin and filter-sterilised through 0.22µm filter. Supplemen ...

Harvest cells and count as for passaging. 1x 107 cells in 1 ml are required for each 1 ml cryovial in 10% DMSO.For example:If 1ml cell suspension obtained from harvest contains 50 x 106 cells/ml.:For ...

Labelling Drosophila pupae with BrdU ITO Kei 1992 OctoberCOLLECTING STAGED PUPAE 1. Prepare a couple of bottles containing late 3rd instar larvae which wander on the botlle wall (110-120 hours after e ...

Single and Double FISH protocols for DrosophilaThis procedure is taken from a recently published book chapter. The reference is: Hughes S. and Krause H.M. (1998) Single and double FISH protocols for D ...

Efficient sporulation of BY4743 requires growth as a patch on freshly prepared GNA presporulation plates at 30°C overnight prior to transfer to liquid sporulation medium. (Note: if GNA is inoculated ...

Freezedown Guidelines What to SaveFor each 96-pack each lab will have yeast representatives from all four strains: BY4741 (or BY4730) BY4742 (or BY4739) BY4743 and the homozygous diploid. It is recogn ...

FEDEX Protocol(Or: How to increase the likelihood that your FEDEX packages reach their intended destination in a timely fashion)While this list seems pedantic so far it only incorporates actual bad ex ...

Alternate Sporulation Method for BY4743 Protocol: 1. Patch cells that have been freshly grown on YPD to freshly prepared GNA pre-sporulation plates for 1 day at 30°C.Repatch to another freshly prepar ...

10X HC (7 Dropout Amino Acid Liquid)2.4 g tyr3.2 g ile2.0 g phe4.0 g glu8.0 g thr4.0 g asp6.0 g val16.0 g ser0.8 g argin final volume of 4L - Autoclave10X YNB58 g YNB (w/o amino acids and w/o ammoni ...

100X YC (7 Dropout Amino Acid Liquid)1.0 g arginine0.5 g aspartic acid0.5 g isoleucine0.5 g phenylalanine0.5 g proline0.5 g serine1.0 g threonine0.5 g valinein final volume of 100 mlstir with low hea ...

COMPLEX MEDIA YPAD medium or Also Called YPD plus AdenineYeast Extract - Peptone - Dextrose plus Adenine medium 6.0 gm yeast extract (Difco) 12.0 gm Peptone (Difco) 12.0 gm Glucose 60 mg adenine hem ...

Yeast Plates Hahn LabLast modified Tue Oct 6 1998Amino Acid Mix (40X) Use 0.6 g/literTyrosine 2 gArginine 4 gSerine 2 gValine 2 gThreonine 4 gIsoleucine 2 gPhenylalanine 2 gAspartic Acid 2 gProline 2 ...