小鼠脂褐质(LF)酶联免疫分析(ELISA)试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定小鼠血清组织匀浆及相关液体样本中脂褐质(LF)含量。实验原理:本试剂盒应用双抗体夹心法测定标本中小鼠脂褐质(LF)水平。用纯化的小鼠脂褐质(LF)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入脂褐质(LF),再与HRP标记的脂褐质(LF)抗体结合,形成抗体-抗原-酶标 ...
大鼠4-羟基壬烯醛(4-HNE)酶联免疫分析(ELISA)试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定大鼠血清,血浆组织及相关液体样本中4-羟基壬烯醛(4-HNE)的含量。实验原理:本试剂盒应用双抗体夹心法测定标本中大鼠4-羟基壬烯醛(4-HNE)水平。用纯化的大鼠4-羟基壬烯醛(4-HNE)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入4-羟基壬烯醛(4 ...
大鼠5α-还原酶Ⅱ酶联免疫分析试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定大鼠血清,血浆,细胞上清及相关液体样本中5α-还原酶Ⅱ的含量。实验原理:本试剂盒应用双抗体夹心法测定标本中大鼠5α-还原酶Ⅱ水平。用纯化的大鼠5α-还原酶Ⅱ抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入5α-还原酶Ⅱ,再与HRP标记的5α-还原酶Ⅱ抗体结合,形成抗体-抗原-酶标抗体复 ...
大鼠Ⅲ型前胶原(PCⅢ)酶联免疫分析(ELISA)试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中Ⅲ型前胶原(PCⅢ)的含量。实验原理:本试剂盒应用双抗体夹心法测定标本中大鼠Ⅲ型前胶原(PCⅢ)水平。用纯化的大鼠Ⅲ型前胶原(PCⅢ)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入Ⅲ型前胶原(PCⅢ),再与HRP标记的Ⅲ型前胶原(PCⅢ ...
大鼠Ⅲ型胶原(ColⅢ)酶联免疫分析试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定大鼠血清,细胞上清及相关液体样本中Ⅲ型胶原(ColⅢ)的含量。实验原理:本试剂盒应用双抗体夹心法测定标本中大鼠Ⅲ型胶原(ColⅢ)水平。用纯化的大鼠Ⅲ型胶原(ColⅢ)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入Ⅲ型胶原(ColⅢ),再与HRP标记的Ⅲ型胶原(Col ...
大鼠APJ酶联免疫分析(ELISA)试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中APJ的含量。实验原理:本试剂盒应用双抗体夹心法测定标本中大鼠APJ水平。用纯化的大鼠APJ抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入APJ,再与HRP标记的APJ抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在 ...
大鼠肿瘤坏死因子α(TNF-α)酶联免疫分析(ELISA)试剂盒使用说明书本试剂仅供研究使用目的:本试剂盒用于测定大鼠血清,组织及相关液体样本中肿瘤坏死因子α(TNF-α)的含量。实验原理:本试剂盒应用双抗体夹心法测定标本中大鼠肿瘤坏死因子α(TNF-α)水平。用纯化的大鼠肿瘤坏死因子α(TNF-α)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入肿瘤坏死因子α(TNF- ...
重度联合免疫缺陷(SCID) 小鼠是美国Bosma证实的属CB - 17 近交系小鼠第16 号常染色体发生隐性突变基因(SCID) 的突变型小鼠1 。纯合的SCID 鼠基因导致小鼠几乎完全丧失T 和B 淋巴细胞的功能 人们便将这类小鼠广泛用于免疫学、肿瘤学的研究。急性早幼粒细胞白血病(APL) 在诱导分化剂全反式维甲酸作用下可向正常粒系分化临床上用全反式维甲酸(ATRA) 治疗APL 虽能引起完 ...
同源重组技术原理:基因敲除鼠技术是上世纪80年代中后期基于DNA同源重组的原理发展起来的,Capecchi和Smithies在1987年根据同源重组(homologous recombination)的原理,首次实现了ES的外源基因的定点整合(targeted integration),这一技术称为"基因打靶"(gene targeting)或"基因敲除"(gene knockout),利用这种E ...
一、常规基因敲除鼠(Conventional Knockout)常规基因敲除是通过基因打靶,把需要敲除的基因的几个重要的外显子或者功能区域用Neo Cassette 替换掉。这样的小鼠其全身所有的组织和细胞中都不表达该基因产物。此类基因敲除鼠一般用于研究某个基因在对小鼠全身生理病理的影响,而且这个基因没有胚胎致死性。二、条件性基因敲除小鼠(Conditional Knockout)条件性基因敲除小 ...
Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI Introduction The following is a simple procedure for determining cell number in preimplanta ...
ContentsEmbryonic Stem Cell Markers Hematopoietic Stem Cell Markers Mesenchymal/Stromal Stem Cell Markers Neural Stem Cell Markers References While stem cells are best defined functionally a number of ...
In Vitro Production of Bovine Embryos (P.J. Hansen Lab Dept. of Animal Sciences University of Florida)This protocol describe procedures for in vitro production in vitro fertilization (IVF) and in vit ...
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C � do not go higher). Add a few drops of 2 N sodium hydroxide until ...
1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947). 2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fasten ...
ABSTRACT Knowledge of the geneological relationships of cells during development can allow one to gain insight into when and where developmental decisions are being made. Geneological relationships ca ...
8 eggs per day day 7- day 13 cut CAM wash in precooled PBS in 10 ml WASH 1 (PBS 5 mM EDTA COMPLETE) on ice cut in pieces in petri dish on ice centrifuge 4 min at 1000 rpm (table top centifuge Falcon 1 ...
1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947). 2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fasten ...
1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forcepsuse another to tear the mesometrium away from the uterus.3. Remove the embryos fr ...
Ovulation is induced in X. tropicalis by injection of human chorionic gonadotropin (hCG). X. tropicalis requires a much smaller dose than does X. laevis. We use basically the same protocol as that des ...