细胞技术

This chapter reviews the theory and methodological developments of on-line concentration techniques for the determination of environmental pollutant samples, such as organic and inorganic compounds in capillary zone electrophoresis (CZE) and also in micellar electrokin ...

Microtubules are required throughout plant development for a wide variety of processes, and different strategies have been evolved to visualize them. This chapter summarizes the most effective of these methods and points out potential problems and pitfalls. We outline the freeze-sh ...

Preservation of Tetrahymena thermophila basal body ultrastructure for visualization by transmission electron microscopy is improved by a combination of high pressure freezing (HPF) and freeze substitution (FS). These methods also reliably retain the antigenicity of cellu ...

The growth and maintenance of almost all cilia and flagella are dependent on the proper functioning of the process of intraflagellar transport (IFT). This includes the primary cilia of most cells in humans that are in interphase or the Go phase of the cell cycle. The model system for the study of IFT is the fl ...

The cytoskeleton is a complex of detergent-insoluble components of the cytoplasm playing critical roles in cell motility, shape generation, and mechanical properties of a cell. Fibrillar polymers−actin filaments, microtubules, and intermediate filaments− are major constit ...

The budding yeast Saccharomyces cerevisiae has many advantages as a model system, but until recently, high-resolution microscopy was not often attempted in this organism. Its small size, rounded shape, and rigid cell wall were obstacles to exploring the cell biology of this model eukaryot ...

During the past decade the use of live cytoskeletal probes has increased dramatically due to the introduction of the green fluorescent protein. However, to make full use of these live cell reporters it is necessary to implement simple methods to maintain plant specimens in optimal growing con ...

Changes in conformation are an important regulatory mechanism for a wide variety of proteins. Proteins whose activity must change in response to external stimuli often undergo dramatic changes in their tertiary structure in a temporally and spatially coordinated manner, resulting ...

Fluorescence microscopy is a non-invasive technique that allows high resolution imaging of cytoskeletal structures. Advances in the field of fluorescent labelling (e.g., fluorescent proteins, quantum dots, tetracystein domains) and optics (e.g., super-resolution techniq ...

Centrosomes are essential organelles that organize the microtubule cytoskeleton during interphase and mitosis. Centrosomes are assembled from tens to hundreds of proteins, but how these proteins are organized into functional microtubule nucleating and organizing centers ...

This chapter describes labeling methods and optical approaches for live-cell imaging of the cytoskeleton and of a specific organelle–cytoskeleton interaction in budding yeast.

Historically, much of our understanding of actin filaments, microtubules and intermediate filaments has come from the study of fixed cells and tissues. But the cytoskeleton is inherently dynamic, and so developing the means to image it in living cells has proved crucial. Advances in confoc ...

Cell migration is a multi-step process that involves sequential changes in the cytoskeleton, cell–substrate adhesion and components of the extracellular matrix. In multicellular organisms, directional cell migration is important for normal development, wound healing and im ...

A key to understanding cytoskeletal mechanisms of eukaryotic cells is found in their internal motility. In many plant cell types, these motile events, termed “cytoplasmic streaming”, are very impressive with rapid movement of organelles over long distances. Like many other features of c ...

Environmental pollutants comprise a variety of compounds from inorganic anions, cations, ionizable organic compounds and moderately hydrophobic organic compounds to highly hydrophobic organic compounds. Correspondingly different separation strategies are requ ...

Low-molecular-weight (LMW) aliphatic amines play a key role in the global nitrogen cycle, are involved in nutrient transfer, and act as buffer in the ecosystem. They are widely used as intermediates in chemical synthesis and were shown to cause occupational asthma. Biogenic amines occur in all ...

Within the last few years, capillary electrophoresis (CE), especially with indirect ultraviolet detection, has successfully been utilized for the analysis of low-molecular-weight (LMW) organic acids in a wide variety of matrices (e.g., food, pharmaceutical, environmental, ind ...

Capillary electrophoresis has recently attracted considerable attention as a promising analytical technique for metal ion separations. Significant advances in various auxiliary separation principles have opened new application areas for capillary electrophores ...

Microorganisms can be considered a bio-colloid. That is, they have a characteristic outer surface that carries, or can carry, a charge. Precisely, differences in the surface can be exploited for separation by capillary electrophoresis (CE). In fact, methods based on CE seem to be very promising b ...

Capillary electrophoresis (CE), especially free-zone CE, offers a relatively simple separation with moderate selectivity based on the mobility of ions in solution. Laser-induced fluorescence (LIF) detection, an extremely sensitive technique, can be coupled with a variety of sep ...