细胞技术

Endocrine tissues like the pituitary, hypothalamus and islets of Langerhans are rich in bioactive peptides. These are used for intercellular signalling and are involved in regulation of almost all physiological processes. Peptidomics is the comprehensive analysis of peptides in ...

A large number of diverse antimicrobial peptides have been found in amphibian skins, and many more remain to be identified. It is sufficiently easy to obtain amounts of gland secretions sufficient for both identification and functional testing of the bioactive peptides. We describe here a s ...

Antimicrobial peptides (AMPs) play an important role in the host’s innate defence system in many organisms. Amphibian skin is expected to be a particularly rich source of novel AMPs. In amphibians, AMPs are produced from precursor proteins via specific cleavage by processing enzymes. While ...

Skin secretions from anurans (frogs and toads), particularly those species belonging to the Hylidae and Ranidae families, are a rich source of biologically active peptides. Cytolytic peptides with broad-spectrum antimicrobial activities and highly variable amino acid sequen ...

Today, commercially available mass spectrometers increasingly meet all the demands of the proteomics community including high throughput, high sensitivity, and significant fragmentation capability for sequence determinations. Therefore, proper sample preparati ...

Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to analyze the peptide complement in the nervous system of a variety of invertebrate animals from leech to Aplysia and many arthropod species, especially insects and crustaceans. Here, we describe a protocol f ...

The term “peptidomics” can be defined as the systematic analysis of the peptide content within a cell, organelle, tissue or organism. The science of peptidomics usually refers to the studies of naturally occurring peptides. Another meaning refers to the peptidomics approach to protein an ...

The central nervous systems of molluscan species contain high levels of structurally diverse peptides that function as neurotransmitters, neuromodulators or neurohormones. Peptide diversity is believed to be a way to increase the information handling capacity of neurons in the c ...

Neuropeptides are important signaling molecules that regulate many essential physiological processes. Microdialysis offers a way to sample neuropeptides in vivo. When combined with liquid chromatography–mass spectrometry detection, many known and unknown neuropept ...

Spider venom contains a complex mixture of components with a large range of molecular masses (0.1–60kDa) exhibiting a diverse array of actions. Most of these components are proteinaceous molecules – biologically active proteins and peptides. Proteomics profiling of spider venoms (t ...

Spider venoms represent invaluable sources of biologically active compounds suitable for use in life science research and also having a significant potential for biotechnology and therapeutic applications. The methods reported herewith are based on our long experience of spid ...

The diversity of insect neuropeptides coupled with the limitations from the small size of the insects themselves combine to make positive identification through peptide sequencing a highly challenging task. The advent of the “soft-ionisation” techniques of MALDI-TOF and electro ...

Affinity peptidomics relies on the successfully proven approach used widely in mass-spectrometry-based protein analysis, where protein samples are proteolytically digested prior to the analysis. Unlike traditional proteomic analyses, affinity peptidomics employs a ...

The tachykinins represent the largest known peptide family and are responsible for a range of pleiotropic functions in both vertebrates and invertebrates. Recent research has shown a diversity of mechanisms such as mRNA splicing, precursor processing and post-translation modif ...

Little is known about peptides that control developmental processes such as cell differentiation and pattern formation in metazoans. The cnidarian Hydra is one of the most basal metazoans and is a key model system for studying the peptides involved in these processes. We developed a novel pep ...

Plant peptides play a number of important roles in defence, development and many other aspects of plant physiology. Identifying additional peptide sequences provides the starting point to investigate their function using molecular, genetic or biochemical techniques. Due to their ...

Phospholipids are amphipathic molecules of great importance and widespread in biological material. They play an essential role in the structure and function of biological membranes. They are classified according to the nature of the “backbone” residue (glycerol or sphingosine), t ...

The most widely used subcellular fractionation techniques (e.g., centrifugation) are dependent on differences in physical parameters between organelles (e.g., size and density). In contrast, immunological techniques rely on biological differences, i.e., the expression of dif ...

The isolation of mitochondria from plant cells that display similar biochemical and morphological characteristics to those observed in vivo requires considerable expertise. Although it is relatively easy to prepare a crude mitochondrial fraction by differential centrifu ...

The membranes of animal cells contain a substantial amount of lipid, which may account for between 20 and 80% of the membrane mass. The predominant lipid components are phospholipids (PL) and cholesterol; with glycolipids, cholesterol esters, glycerides, and free fatty acids as minor const ...