细胞技术

树突状细胞(ＤｅｎｄｒｉｔｉｃｃｅｌｌＤＣ)是机体免疫系统中最强有力的一种专职的抗原呈递细胞(ＡＰＣ)在免疫应答的启动、调控上起着关键的作用。现代抗肿瘤免疫疗法利用ＤＣ负载肿瘤相关抗原(ＴＡＡ)制成的肿瘤疫苗(瘤苗)在转移性前列腺癌、恶性黑色素瘤、多发性骨髓瘤和慢性髓性白血病(ＣＭＬ)等几种恶性肿瘤的治疗上已取得喜人的效果。现对树突状细胞的生物特性、来源、抗瘤机制、肿瘤疫苗疗法等进行综述。1　ＤＣ ...

首先，它肯定不同于原代细胞系的，最大的区别，原代的心肌细胞基本不能分裂，而H9C2可以分裂，最大的区别了吧！原代心肌细胞会跳动，你的H9C2不会吧。虽然都是梭形的，但是H9C2来源于胚胎期心脏，而心肌细胞一般用新生大鼠分离获得。 H9C2与原代心肌细胞有那么大区别，当然不能替代它，所以在研究方向上，你要根据自己的实验需求选择自己合适的细胞，一般来讲呢，如果你研究与心脏相关的信号通路，我建议你还是选 ...

Human Astrocytes Astrocytes make up the majority of the cells in the mammalian brain. They are the most variable in type most intimately associated with all parts of neurons and thus most functionally ...

A general protocol for staining cell for cytometry analysis General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Di ...

When an irreplaceable culture becomes contaminated researchers may attempt to eliminate or control the contamination. First determine if the contamination is bacteria fungus mycoplasma or yeast. Isola ...

Authentication Techniques Whatever the scope of work to be carried out it is important to know that the work is being conducted using the correct reagents. This is no less important for cell cultures ...

Chemotaxis Assayby Melissa Swope Purpose The purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cell type. Chemota ...

小鼠胚胎成纤维细胞的富集 1、给13-14天的孕鼠注射大约0.5ml阿佛丁。当鼠麻醉后，实施断颈法处死小鼠。 2、用70％乙醇擦拭腹部，把皮肤向后拉，暴露出腹膜。用消毒过的工具，剪开腹壁以暴露出子宫角。将子宫角移到10cm的皿里。用10ml不含钙镁离子的PBS洗三遍。 3、用剪刀剪开每一测的胚囊并将胚胎移到培养皿中。 4、用两副钟表镊子将胎盘和膜与胚胎分离开，分离后切除内脏（所有深色的东西）。将胚 ...

目的 探讨阿司匹林对人γδT细胞杀伤消化道肿瘤细胞株的影响。方法 用异戊烯焦磷酸法体外扩增人外周血γδT细胞。用不同浓度的阿司匹林诱导γδT细胞和消化道肿瘤SGC-7901、SW-1990、SW-480、SW-1116、LOVO细胞株，用MTT法检测阿司匹林对这些细胞的抑制率和用乳酸脱氢酶法测定γδT ...

1、pcDNA3.1＋-gD的线性化: pcDNA3.1＋使用说明书推荐了以下几个酶作为线性化酶(BglⅡ MfeⅡ Bst1107Ⅰ Eam1105Ⅰ PvuⅠ ScaⅠ SspⅠ)通过DNAMAN分析gD序列发现其带有MfeⅡ酶切位点。 2、转染前一天在60mm的dish中接种8×105个细胞加入5ml培养基(含血清不含抗生素)37℃5%CO2培养至转染时要求细胞40%-80%汇合 ...

PROCEDURE for preparation of activated platelets 1. Centrifuge tube of freshly drawn HEP or EDTA blood at 600 rpm (75xg) for 20 minutes. 2. Remove all of platelet rich plasma (top layer) and place i ...

Internalization of receptor is measured as the reduction of receptors on the plasma membrane surface. Surface receptors are labeled with fluoresent antibodies which is in turn measured using a flow cy ...

Accuracy of manual counts with an hemacytometer depend on: accurate mixing of the sample (no bubbles!) number of chambers counted number of cells counted (practical: 200-500 per 0.1 mm3) The chamber o ...

Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note: Trypan blue has a greater affinity for serum proteins than for cellular protein. If the back ...

Counting cells by the use of a hemacytometer is a convenient and practical method of determining cell numbers in the case that the Coulter counter is out-of-order temporarily. (It is not that bad.) T ...

Materials Suspension cultures set up from Exercise 12.8 Sterile transfer pipettes Materials for viability counting (Exercise 12.7) Procedure After 12 hours aseptically remove 0.1 ml from each of the t ...

Load both sides of the hemacytometer with the cell suspension - covering both counting grids. Count the number of cells in 5 out of 9 squares from each counting grid (count a total of 10 squares). Div ...

A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the chamber floor. The counting chamber is etched in a ...

We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a result most of our incub ...

1. embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c. 2. metanephroi and associated ureteric buds are microdissected and placed in holding medium (L15 medium supplemented ...