细胞技术

Preparation of Mitochondria from Rat Liver Rat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawley albino male rats for our studies. Female rats ...

Recommended Experiments with Isolated Mitochondria Each oxygraph setup will consist of two stations each with dissolved oxygen chamber and chart recorder connected to a two-channel oxygen monitor. Ple ...

Mitochondria Structure and Function - Theoretical DetailsIn an attempt to be concise and understandable introductory level courses and textbooks frequently present concepts that are technically correc ...

Crystal Violet AssayThis is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dye in this assay crystal violet ...

Phycoerythrin conjugation protocolDavid's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. ReagentsAbAntibody about ...

Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith AD; Winkler H. A simple method for the isolation of ad ...

Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith AD; Winkler H. A simple method for the isolation of ad ...

Pheromone Halo AssayREFERENCE: Hoffman G. Garrison T. R. and Dohlman H. G. Analysis of RGS proteins in Saccharomyces cerevisiae Methods Enzymol.344:617-631 2002. -Use sterile technique and sterile s ...

Pheromone Halo AssayREFERENCE: Hoffman G. Garrison T. R. and Dohlman H. G. Analysis of RGS proteins in Saccharomyces cerevisiae Methods Enzymol.344:617-631 2002. -Use sterile technique and sterile s ...

SAPK/Jun kinase assaysPreparation of cell lysate: 1. For cells in suspension grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1 K for 5 min) and obtain a co ...

SAPK/Jun kinase assaysPreparation of cell lysate: 1. For cells in suspension grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1 K for 5 min) and obtain a co ...

In Vitro T Cell ActivationIntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequence of TCR activation i ...

Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cell cultures or isolated cell lines that are depende ...

Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are critical in studies of cytokine function. The followi ...

Overview This assay uses a simple apparatus to study chemotaxis of leukocytes or other migratory cells. The apparatus consists of two multi-well chambers separated by a filter containing pores of uni ...

Overview This assay uses a simple apparatus to study chemotaxis of leukocytes or other migratory cells. The apparatus consists of two multi-well chambers separated by a filter containing pores of uni ...

overviewThe Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavior of individual migrating cells can be studied by ...

overviewThe Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavior of individual migrating cells can be studied by ...

MTT 5-dimethylthiazol-2-yl)-25-diphenyltetrazolium bromide assay first described by Mosmann in 1983 is based on the ability of a mitochondrial dehydrogenase enzyme from viable cells to cleave the tetr ...

Thymidine-Incorporation Assay for Rat-1a cells Overview This method of Peter Coward Ph.D. in the Conklin Lab was used in Coward et al (1998) Controlling signaling with a specifically designed ...