细胞技术

作者：陈文庆 王建超 刘华杰 高飞 张韧（北京清大天一科技有限公司 102200 ）（《中国兽药杂志》 2010.44 （ 10 ）： 37-41 ） 【摘要】 采用反应器全悬浮培养BHK21细胞生产口蹄疫病毒与微载体悬浮培养Vero细胞生产狂犬病毒分别与相应的转瓶培养工艺生产案例对比分析，比较悬浮培养工艺与转瓶培养工艺的生产效益。分析显示，与转瓶培养工艺相比，反应器 ...

一、细胞培养步骤 细胞原代培养步骤：http://www.biomart.cn/experiment/451.htm 细胞传代培养步骤：http://www.biomart.cn/experiment/505.htm 更多细胞培养实验：http://www.biomart.cn/experiment/allExperiment.htm 二、细胞培养常见问答 1、加到培养基中的血 ...

iPS细胞在治疗血液疾病的应用Xu等将iPS细胞诱导分化为内皮前体细胞，然后移植到患有血友病小鼠的肝脏中，使病鼠出血不止的症状得到了有效地改善。Hanna等人将患病小鼠尾尖成纤维细胞重编程为 iPS细胞，然后通过同源重组的方法用人野生型 p A.珠蛋白基因替代了 pass珠蛋白基因接着把遗传修饰后的 iPS细胞定向分化为造血祖细胞 (HPs)并将纯化后的 HPs移植入 hps /llps雄性小鼠中 ...

四因子作用那么是否可以直接应用相关转录因子对体细胞进行重编程而逆转终未分化细胞的发育潜能 进而表现出ES细胞那样的多潜能性呢？Takahashi和Yamanaka{4}选择24种在小鼠早期胚胎ES细胞或者肿瘤细胞中丰富表达的转录因子 通过筛选、组合 发现用Oct4 Sox2 c-Myc和Klf4 4个因子(这4个因子也被称为Yamanaka因子)可以有效地将胎鼠成纤维细胞(mouse embryo ...

iPS细胞的概述IPS细胞是通过导入特定的转录因子可将分化的体细胞重编程为诱导性多能干细胞（Induced pluripotent stem cellsiPS cells）。特定的转录因子为 Oct4 Sox2 c-Myc和Klf4 4个因子(这4个因子也称为Yamanaka因子)可以有效地将胎鼠成纤维细胞(mouse embryonic fibroblast MEF细胞)和小鼠尾尖成纤维细胞诱导 ...

经过了近5年既漫长又短暂的发展，iPS细胞技术已经取得了举世瞩目的进展。一个个突破性的成果既给我们带来了喜悦，也带来了新的挑战，细胞重编程有望迎来一个新的研究浪潮。尽管iPS细胞有着诱人的应用前景，然而，未来iPS细胞的研究也面临着许多亟需解决的问题：第一，效率问题。目前，诱导产生iPS细胞的率仍然很低，这与基因导人的方式整合位点、表观遗传学等多种因素有关。这已成为制约iPS从实验室走向临床的最大 ...

Stock Solutions1. 2M Cacl2 Merck. Filter (to remove particulates) autoclave and store at -20¡ in aliquots.2. 2 x HEBS gm/500ml NaCl 8.0 KCl 0.38 Na2HPO4.7H2O 0.19 glucose 1.0Hepes 5.0Adjust pH to 7.05 ...

Chemotaxis Assayby Melissa Swope 3/12/2000 PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cell type. ...

一、流式细胞术发展简史 　　流式细胞术（Flow Cytometry FCM）是一种可以对细胞或亚细胞结构进行快速测量的新型分析技术和分选技术。其特点是：①测量速度快，最快可在1秒种内计测数万个细胞；②可进行多参数测量，可以对同一个细胞做有关物理、化学特性的多参数测量，并具有明显的统计学意义；③是一门综合性的高科技方法，它综合了激光技术、计算机技术、流体力学、细胞化学、图像技术等从多领域的知识和成 ...

CELL ADHESIONProcedure:1) Dissolve/dilute coating substrate in ddH2O at 4 C. A common working dilution for the laminin-1 positive control and BSA (Sigma A8412) negative control is 40 ug/ml. For SN-pep ...

Cell Cycles - IntroductionThe onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes and the whitefish has o ...

Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC PlatesRonald L. Schnaar~Professor Johns Hopkins University Medical School Baltimore Maryland 21205This procedure all ...

PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isolated nuclei. The PI intercalates into the major gr ...

Immunofluorescent Localization of TubulinLEVEL II Materials Coverslip cultures of an appropriate monolayer cell line Phosphate buffered saline (PBS) Acetone/Methanol (absolute) in a 50:50 volume mixtu ...

Isolation of Actin and Myosin FilamentsLEVEL III Materials Relaxing Solution0.1 M KCl0.001 M MgCl5 mM ATP0.016 M NaHPONaHPOAdjust pH to 7.30.05 M Sodium phosphate buffer pH 7.00.001 M EDTA (Ethylene d ...

Isolation of Microtubules (Bovine Brain)LEVEL II Materials Freshly removed bovine brain 2Wire sieve (tea strainer)Microtubule buffer (MT buffer)0.1 M MES (2-(N-Morphilino)ethanesulfonic acid)1 mM EGTA ...

Em observations of microsomesLEVEL I Figure 7.3 Isolated microsomes Figure 7.4 TEM of hepatocyte MATERIALS 1% Glutaraldehye (GTA)1% Osmium tetroxideEpoxy or vinyl resin for TEMTEM photomicrograph of ...

DNA Fragmentation Assays for Apoptosis ProtocolProtocol I: Triton X-100 Lysis Buffer In 96 flat-wells plate incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration of effect ...

Staining Methods for cell death Z. Xia 10/2/95The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green alive cells; P.I. (propidium iodide)-red dead cells ...

In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809) modified by Josiah N. Wilcox andJosé C. Rodriguez - Emory University - April 1996Protocol for Fro ...