细胞技术

This chapter describes the application of capillary electrophoresis (CE) to pharmaceutical analysis. The areas of pharmaceutical analysis covered are enantiomer separation, analysis of small molecules such as amino acids or drug counter-ions, pharmaceutical assay, related ...

This chapter reports an overview of the recent advances in the analysis of neutral sugars by capillary electrophoresis (CE); furthermore, some relevant reviews and research articles in the field are tabulated. Comparison of CE with chromatography is also presented, with special atten ...

This chapter illustrates the usefulness of capillary electrophoresis (CE) for the analysis of sugar acids, that is, monosaccharides and lower oligosaccharides carrying carboxylate, sulphate or phosphate groups. In order to provide a general description of the main results and cha ...

Capillary electrophoresis (CE) applications to charged polysaccharides are briefly reported. A simple procedure is presented to determine the esterification degree of a hyaluronan derivative. In this case, the degree of substitution was as low as 14%. The molecular weight distribu ...

This chapter illustrates the usefulness of capillary zone electrophoresis (CZE) coupled to high-resolution electrospray ionization quadrupole time-of-flight mass spectrometry for the single-step desalting, and separation, as well as characterization of oligonucle ...

This chapter reports an overview of the analytical techniques used to perform genetic analysis of polymerase chain reaction products using capillary electrophoresis. Three separate but related techniques are described: the separation of native DNA with detection using fluore ...

This chapter illustrates the usefulness of capillary electrophoresis (CE) for the detection of gene mutation, i.e., point mutation, methylation, and microsatellite analysis. In order to provide a general description of the main results and challenges in the field, some relevant appli ...

Urinary 8-hydroxy-2′-deoxyguanosine (8OHdG) is an excellent marker of oxidative DNA damage. Until now, urinary 8OHdG has been measured by high-performance liquid chromatography with electrochemical detection. A simple and sensitive method for the analysis of urinary 8OHdG by ca ...

Capillary electrophoresis (CE) is an efficient tool for amino acid (AA) analysis. However, its role can be fully accounted for only by examining the applications on real matrices. Methods must be successfully transferred into working environments for use by non-CE experts before their pow ...

Peptides are an important class of analytes in chemistry, biochemistry, and food chemistry as well as medical and pharmaceutical sciences. As a high-resolution technique, capillary electrophoresis (CE) is well suited for the analysis of polar compounds such as peptides. In addition, CE is ...

Capillary Electrophoresis (CE) has become an accepted method for the separation of inorganic and organic ions. Usually, direct and indirect optical detection methods are used in conventional CE. However, with contactless conductivity detection, much better detection limits in the ...

This chapter describes the basic principles of protein analysis by capillary electrophoresis, and provides an overview of the literature and thus a comprehensive summary of special topics in this field. The “Materials and Methods” section includes the main experimental points to be ta ...

Capillary electrophoresis (CE) is a very efficient tool for separating and characterizing synthetic polymers, copolymers, and polyelectrolytes. Different modes of CE (free solution capillary electrophoresis , entangled polymer solution CE , capillary gel electrophores ...

Umbilical cord blood (UCB) is gaining more prominence in recent times as a source of non-embryonic multipotent stem cells. Global annual human birth rate (100 million) presents UCB as the largest non-controversial stem cell source, with an added advantage of naive immune status. Cord blood st ...

Human cord blood (CB), collected from the postpartum placenta and umbilical cord, has been identified as a rich source of hematopoietic stem cells (HSCs) and provides an alternative to bone marrow or mobilized peripheral blood transplantation. However, the major restriction of CB transp ...

Hematopoiesis is the process by which stem cells divide and differentiate to produce the multiple types of mature cells found in blood. The process begins in early embryonic development and continues throughout adult life, primarily in the bone marrow. Various in vivo and in vitro assays have b ...

In organ transplantation, major efforts are currently developed to minimize or even to withdraw immunosuppressive drugs. Different protocols have therefore been proposed to induce graft tolerance, that is, the survival of an allograft in the absence of continuing immunosuppress ...

Mesenchymal stem cells (MSCs) are multipotent progenitor cells isolated by various relatively easily accessible tissues, such as bone marrow and cord blood. MSCs gained attention because of their ease for in vitro expansion together with their multilineage potential. More recentl ...

Intact or genetically manipulated mesnechymal stem cells (MSCs) are being considered an important cell source for developing human cell-based therapeutic approaches. For applications in which transient, high-level expression of the transgene is necessary, adenovirus vect ...

Mesenchymal stem cells or marrow stromal cells (MSCs) represent a multipotent adult cellular population with immunomodulatory functions. In the adult human body, they are present in various niches, but their main source is bone marrow (BM). The regeneration capability of MSCs, their ease ...