Human hematopoietic tissue represents a complex developmental system that is closely regulated to ensure the maintenance of appropriate levels of circulating blood cells. At least nine distinct lineages of cells at various stages of maturation have been described (1). Substantial ...
My laboratory is involved in characterizing the behavior of cultured fibroblasts established from skin biopsies of normal boys and those affected with Duchenne muscular dystrophy. We are also interested in the properties of fibroblasts obtained from female carriers of this X-linked ...
Erythroid progenitor cells have been classified into three groups of increasing maturity: the primitive burst forming unit (p-BFU-E), the mature burst forming unit (m-BFU-E), and the erythropoietin responsive colony forming unit (CFU-E). This classification is based on their time of ma ...
Bone marrow cells from a number of animal species have been used extensively in liquid and semisolid cultures to study hemopoiesis and to produce functional mature cells and factor-dependent cell lines (for review see ref. 1). Neutrophils and macrophages are produced without added growth f ...
It is appropriate that a chapter on cytogenetic techniques for studying leukemic tissue appears in a series on molecular methods since these subjects are closely related, both in history and in application. It is through molecular methods that the significance of specific recurring chro ...
The so-called hematopoietic stem cells from mouse and human bone marrow can be induced in vitro to form colonies, provided that the appropriate hormones (colony stimulating factors, CSFs) and culture conditions have been selected. Such hematopoietic colony cultures are of interest for ...
Cinematography commenced as a scientific technique used as a system for “slowing down” observed movement. Marey in 1888 (1) constructed, following a number of other ideas, a “Chambre Chronophoto-graphique,” which had practically all the elements of the modern cine camera. With this he made s ...
Despite the enthusiasm of the first investigations of cell ultrastructure, morphological studies have since lost some of their importance for biomedical research. The development of quantitative biochemical methods has been the cause of this reduced interest in morphology. Bio ...
We describe a method to derive cell lines and clones from cells of the murine midgestation aorta-gonads-mesonephros (AGM) microenvironment. We start from subdissected AGM regions in “explant-” or “single-cell suspension”-type cultures from embryos transgenic for tsA58, a tempera ...
Normal human and mouse bone marrow is composed of hematopoietic and nonhematopoietic cells. The latter have also been termed stromal cells, microenvironment cells, colony-forming-unit fibroblasts (CFU-F), and mesenchymal cells. These cells were originally thought to provide an ...
The architecture of mammalian skin incorporates an outer layer of stratified epithelium. This enables the organism to conserve internal homeostasis and maintain protection from adverse environmental exposure. The keratinocyte is the cell primarily responsible for this stru ...
As our knowledge of the species differences in drug metabolism and drug-induced hepatotoxicity has expanded significantly, the need for human-relevant in vitro hepatic model systems has become more apparent than ever before. Human hepatocytes have become the “gold standard” for eva ...
Primary rabbit kidney epithelial cell cultures can be obtained that express renal proximal tubule functions. Toward these ends, renal proximal tubules are purified from the rabbit kidney by the method of Brendel and Meezan. To summarize, each kidney is perfused with iron oxide, which becom ...
Normal human mammary tissue is composed of a glandular epithelium embedded within a fibrous and fatty stroma. Collagenase and hyaluronidase digestion of normal reduction mammoplasty specimens followed by differential centrifugation yields a suspension of single cells and ce ...
Murine embryonic day 14 or E14 neural stem cells (NSCs), first isolated and characterized as a stem cell in culture, are a unique population of cells capable of self-renewal. In addition, they produce a large number of progeny capable of differentiating into the three primary phenotypes—neuro ...
Cell culture is an invaluable tool for investigators in numerous fields. It facilitates analysis of biological properties and processes that are not readily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, re ...
Isolated cardiomyocytes are a prerequisite to study the biology of cardiomyocytes. Efficient isolation is difficult, as these cells adhere firmly together in the heart and do not divide. Therefore, any experiment is restricted to the amount of calcium-tolerant, rod-shaped cardiomy ...
Leukocyte recruitment from the vasculature occurs under conditions of hemodynamic shear stress. The parallel-plate flow chamber apparatus is an in vitro system that is widely used to study leukocyte recruitment under shear conditions. The flow chamber is a versatile tool for examini ...
The purpose of this chapter is to describe the isolation techniques that result in pure cultures of human vascular endothelial cells from the umbilical vein and umbilical cord blood. We first describe the isolation of human umbilical vein endothelial cells (HUVECs). Additional protoco ...
The protocol for isolation of side population (SP) cells was originally established for murine bone marrow hematopoietic stem cells (HSCs), but it has also been adapted for other species and tissues. This purification strategy offers a simple and reproducible strategy to obtain a highly ho ...
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