生物化学技术

As insects have an open circulatory system the hemolymph can be simply collected through an incision in the body wall. Most conveniently you should cut a leg and let the blood drip into a chilled glas ...

Synthesis reaction:1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1.1/1.3 in 5 ml of methanol.2) Allow reaction to proceed at room temperature for atleast 18 hours in the dark.3) ...

NOTE - Use arachidonic acid from Biomol (# FA-003)1) Make up the appropriate concentration of AA in 100% ethanol.2) Add 1µl of phenol red solution.-- The solution should appear yellow since the free a ...

1. Make standards using sodium phosphate at the following uM concentrations: 0 2 5 7 10 20 40 60 and 80. Use the screw top glass tubes.2. Dry the samples and standards under nitrogen evaporator (Rm 20 ...

PROCEDURE1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible)2. Add the same volume of a 1M KCl 0.2M Phosphoric acid solution and v ...

ReagentsAshing buffer:10 g Mg(NO3)2 100 ml EtOH1.5 N HCl stock:119.7 ml concentrated HCl (11.6M @ 36% by weight)880.3 ml H2O1 N Sulfuric acid stock:28.9 ml concentrated sulfuric acid (18M/36N @ 96% by ...

Materials:All glassware must be acid washed rinsed thoroughly with water then rinsed with acetone and dried.Redistill acetone.Recrystallizing BOG:1) Add 5 grams of Octyl-b-D-glucopyranoside (bOG = Cal ...

I. Lower chloroform phase:1) Dry on rotovapor system with house vacuum lines. It is not necessary to dry sample completely but sufficiently to yield a final volume of ~2 mls.II. Upper aqueous phase:1) ...

Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer of adsorbent material which has been applied to a ...

During this week you will analyze the fatty acid composition of the individual lipid fractions recovered from the TLC plate. Gas chromatography is a very sensitive method for the separation and quanti ...

1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time with room temperature PBS.3) Resuspend final pellet in an appropriate volume of prewarmed ...

Lipid Extraction1) Following the appropriate time of treatment transfer 4.5 ml into each of two duplicate glass pyrex tubes and maintain on ice.2) Spin down cells in the table top centrifuge at 1200 r ...

Materials Tubulin (5 mg/mL) 100 mM Mg・GTP 4 mM Taxol in DMSO PM = 100 mM PIPES pH 6.82 mM EGTA1 mM Mg2SO4 Motor protein (95% purity; 15-20 µM) Cuvettes (200 µL volume 10 mm path length) 0.5 M Tris-OAc ...

Materials Purified Motor Protein 20-80 µM Nucleotide Mix = 50 mM Mg・ATP gamma-32P-ATP to give 5 000 - 10 000 cpm/nmol 10 mM HEPES pH 7.2 1 mM EGTA 1 mM MgCl2 5% Norit A activated charcoal in 50 mM NaH ...

Cellulase enzymes show activity during the ripening of some fruits where their effects on cell walls results in softening of the fruit. In cases of programmed cell death such as the formation of aeren ...

To assay 17 b-HSD activity in lysates cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media Inc. Lavellette NJ) frozen on dry ice and stored at ...

PurposeTransient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line w ...

常用生化试剂货号品 名规 格价 格(元)备 注B1005Acridine Orange1/2 ZnCI2 (吖啶橙)5g/10g190/320Sigma分装B1006Acrylamide (电泳级丙烯酰胺)100g/500g120/500Sigma分装B1007AcrylamideUltra Pure 丙烯酰胺(超纯)100g/200g140/260Amresco分 ...

Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound originally described as a selective inhibitor of PKCδ can inhibit many other PKC-unrelated kinases and ...

Many species of streptococci secrete and use a competence-stimulating peptide (CSP) to initiate quorum sensing for induction of genetic competence bacteriocin production and other activities. These si ...