LIPID EXTRACTION
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827
| PROCEDURE |
| 1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible) |
| 2. Add the same volume of a 1M KCl, 0.2M Phosphoric acid solution and vortex again |
| 3. Centrifuge sample at 10,000 xg for 2 minutes |
|
4. Collect the bottom phase (contains lipids) |
