DNA实验技术

CaCl2 Competent Cells Description: Competent cells are able to take up DNA.... Competent cells CaCl2 method E. coli long protocol Keywords: competent cells; E. coli; calcium chloride; Zappe CaCl2 com ...

Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperature AgaroseReference: Favre D. 1992. Biotechniques vol. 13Cut out slice containing DNA smallest size possible. Estimate ...

ISOLATION OF DNA FROM AGAROSE GELS WITH DEAE PAPER MATERIALS:The DEAE paper can be used as supplied by Schleicher and Schuell however the binding capacity can be increased by washing the paper in 10m ...

ISOLATION OF DNA FROM AGAROSE GELS WITH DEAE PAPER MATERIALS:The DEAE paper can be used as supplied by Schleicher and Schuell however the binding capacity can be increased by washing the paper in 10m ...

Isolation of DNA from Agarose Gels (Paper Slurry Method) This procedure isolates DNA from agarose gels by filtration through a filter-paper column. The column is made in a 500 µL tube from a slurry of ...

An inexpensive alternative to glassmilk for DNA purificationPreparation of Silica1. Suspend 5 g of silica (Sigma S-5631) in 50 ml of PBS. 2. Allow the silica to settle for 2h. 3. Discard the supernat ...

Electroelution of agarose fragmentsElectroelution buffer1 M Tris pH 7.5 12.0 mls0.5 M EDTA 0.24 mls1 M NaCl 3.0 mlsqs to 600 mls dH2OAcetate cushion 3 M NaAcetate pH 4.8 480 ul0.1 % Bromphenol Blue 40 ...

Electroelution of agarose fragmentsElectroelution buffer1 M Tris pH 7.5 12.0 mls0.5 M EDTA 0.24 mls1 M NaCl 3.0 mlsqs to 600 mls dH2OAcetate cushion 3 M NaAcetate pH 4.8 480 ul0.1 % Bromphenol Blue 40 ...

DNA PrecipitationPhenol (removes protein)add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol) vortex spin 2 minutes at 12000 rpm 4°C transfer supernatant to a fresh tube (avoi ...

Recovery of DNA from LMP (Low Melting Point) Agarose Gel1. Separate DNA fragments through an LMP agarose gel containing ethidium bromide (0.5 microgram/ml). 2. Detect DNA by irradiating the gel with l ...

Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium acetate pH 5.2 (final con ...

Improved Alcohol Precipitation of DNAECKDescription========This method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley 1985). It is a useful way to avoid coprecipitation of ...

Improved Alcohol Precipitation of DNAECKDescription========This method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley 1985). It is a useful way to avoid coprecipitation of ...

Sephadex G-50 spun column purificationSpin column purification can be used to change buffers without a concomitant change in solution volume to remove protein contaminants or to purify plasmid DNA sui ...

1. Prepare or obtain Buffered phenol pH 8. Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation. This also identifies the organic phase as yellow-colored.2. Combine DNA sample with an equal volum ...

Preparation of linear polyacrylamide used as carrier in ethanol precipitation of nucleic acidsLinear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with eth ...

DNA Extraction MethodsPhenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl 10 mM EDTA 50 mM Tris-HCl pH 8). 2. Incubate The sand fly ho ...

DNA Extraction MethodsPhenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl 10 mM EDTA 50 mM Tris-HCl pH 8). 2. Incubate The sand fly ho ...

Rapid Method for Preparation of Genomic DNA from P. aeruginosa Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37° ...

Rapid Method for Preparation of Genomic DNA from P. aeruginosa Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37° ...