DNA实验技术

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c55"; ...

1) Prior to any ligation reaction you should always run one gel in which purified insert and cut backbone are run side by side preferably beside a known amount of cut DNA.You can then use this t ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c55"; ...

This a brief protocol for plasmid mini preparation.

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c55"; ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f ...

10X TBE, per liter Sequencing dye ------------------- ---------------- 108g Tris base 80% formamide 55g boric acid 10mM NaOH 40ml 0.5M EDTA 1mM EDTA 0.1% xylene cyanol 10% APS 0.1% bromophenol blue ------------------- dissolve 0.1g ammonium persulfate in 10ml dH2O and store frozenGEL COMPOSITIONS per liter 6% 8% 20% ------------------------------------------------ acrylamide 57g 76g 190g bis acrylamide 3g 4g 10g urea 500g 500g 500g 10X TBE 100ml 100ml 100ml ddH2O 300ml 300ml 300ml

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c55"; ...

一、质粒的提取及鉴定 （一）质粒的提取及鉴定 1、收获细菌 （1）将2ml含相应抗生素的LB液体培养基加入到通气良好的15ml的试管中，接入一单菌落，于37℃剧烈振荡培养过夜。 （2）将1.5ml培养物倒入1.5ml离心管中，用台式离心机于4℃以12000g离心5min，将剩余的培养物贮存于4℃。 （3）吸尽培养液，使细菌沉淀尽可能干燥。 2、碱裂解法小量抽提质粒 （1）将细菌沉淀重悬于100&m ...

This procedure is used to analyze inserts in pUC-derived plasimids. 1. Suspend E. coli colonies harbouring plasmids in 50 microliters of TE. 2. Incubate for 5 min at 95 degrees or in boiling water. 3. ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c5 ...

Immediately before indcution it is advisable to test the culture to determine the fraction of cells that still carry the target plasmid. This in ...

载体（vector）是指运载外源DNA有效进入受体细胞内的工具。载体同外源DNA在体外重组成DNA重组分子，在进入受体后形成一个复制子，即形成在细胞内能独自进行自我复制的遗传因子。因此，作为载体应该满足以下几方面的要求：①有某种限制酶的一个切点，最好是有许多种限制酶的切点，而且每种酶的切点只有一个；②外源DNA插入后不影响载体在受体细胞中进行自我复制，载体应对受体细胞无害，以及载体能接纳尽可能大的 ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f ...