DNA实验技术

1 目的 ：DNA 抽提标准化操作规范适用于HLA试验中的DNA 抽提操作。 2 适用范围 ：DNA 抽提实验室 3 依据 ： DNA 抽提标准化操作规范 4 引用文件 ：德国BAG公司（以下简称BAG）《Instructions for use EXTRA-GENE I》 5 程序 5.1试剂准备 5.1.1 BAG® EXTRA-GENE I 该试剂盒包括3部分：Solution 1 ...

1.Prepare or obtain Buffered phenolpH 8.Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation.This also identifies the organic phase as yellow-colored. 2.Combine DNA sample with an equal volume of ...

This is a brief overview of how a Southern blot (more formally called an DNA blot)is performed and what type of data you can obtain form one. Figure 1.Southern blots allow investigators to determine ...

1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.Locate bands with a hand-held long-wave UV lamp. 2.Slice the gel with a razor blade above and below the bands of interes ...

hieverybody. i want extract DNA from cryopreserved clotted human blood.the smaple has been storaged 5 years in -20 degree. who is an expert on this topic . i need your help.i need the details. my emai ...

Hi! Would anyone out there be able to tell as to why alchohols like isopropanol or ethanol are used to precipitate nucleic acidsand alsowhy is the precipitation done at low temperatures ramesh -rames ...

A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves (See also 1)Take one medium sized leaf or half a large lea ...

1. Pick pl ...

实验步骤： 1、Inoculate from an overnight grown in LB.从培养过夜的LB平板上挑取单菌落 。 2、Grow in 250 ml "SOB" at 18℃ until OD600 = 0.6.(0.3)接种于250ml SOB，18度培养至OD＝0.6。 3、On ice for 10 minutes.菌液置冰上10分钟。 4、Spin ...

1 导论 聚合酶链式反应（PCR）以其优越性极大地影响到了分子生物学的几乎所有领域，并以其基本程序和DGGE（denaturing gradient gel electrophoresis），开发出多种检测核苷酸变异的方法。RAPD（Random Amplifed Polymorphic DNA）就是其中的一种，它是1990年美国杜邦公司科学家J.G.K.Williams和加利福尼亚生物研究所J ...

Cepko/Tabin Lab Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/S6.html Superfrost plus slides and standard slides can be silinized but I have found that superfrost slides work be ...

This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria we've tried. 1.Pellet cells from 10 ml overnight cultures in BHI or LB and wash in 5 ml ...

1.Pour a vertical acrylamide gel using TEA buffer.A 4 % non denaturing gel is correct for most applications. 2.Run out DNA fragments.For fragments greater than 500 bprun the xylene cyanol dye to the b ...

对于神经科学中分子生物学部分，特别是相关疾病方面的研究手段并不是很多。特别是对于多个因素的作用，现在的研究手段还比较稚嫩，对于DNA 的初步大规模分型无疑是一种好的方法。AFFY用基因来检测SNP达到了高通量的目的，非常适合于第一步的筛查。 复杂DNA 的大规模基因分型 Nature Biotechnology Volume 21 Number 10 October 2003 以复杂的人类表型的 ...

原理 基因组步行技术是一种新发展的利用已知序列(cDNA或基因组DNA)从基因组中获得基因的上游(如启动子)或下游序列的方法。其原理如下：首先利用不同的具有平末端的 DNA 限制性内切酶消化基因组 DNA，然后将预先设计好的 DNA 接头连接在 DNA 的两端这样的一组两端带有接头的 DNA 片段就称为所谓的无载体的 DNA 文库；根据接头和目的基因的序列设计两组引物，以上述的DNA为模板，先以外 ...

This is a modification of the procedure in Short Protocols (1-411-45) 1.Prepare a 50 ml liquid lysate: A.mix 2xlO8 E.coli cells with 100μl phage (from one picked plaque in 500μl SM)and 100μl ...

实验目的 学习和掌握限制性内切酶的特性 掌握对重组质粒进行限制性内切酶酶切的原理和方法 并理解限制性内切酶是DNA重组技术的关键工具。 相关基础知识 限制性核酸内切酶：是一类能识别双链DNA分子特异性核酸序列的DNA水解酶。它是基因工程中用于体外剪切基因片段的重要工具酶。 上世纪七十年代，当人们在对噬菌体的宿主特异性的限制-修饰现象进行研究时，首次发现了限制性内切酶。首批被发现的限制性内切酶包 ...

酶 最适温度 37℃%活性 Apa I 25℃ 10undefined ApeK I 75℃ n ...

(adapted from Bruce A.RoeDepartment of Chemistry and BiochemistryThe University of OklahomaNormanOklahoma 73019 broe@ou.edu) Phenol extraction is a common technique used to purify a DNA sample (1).Typ ...

Materials: 1.TE solution o10 mM Tris (pH to 7.5) o1 mM EDTA (pH to 8.0 to dissolve) 2.Frozen agarose gel piece containing the desired DNA fragment Supplies: 1.Micropipetter and tips 2.Microcentrifuge ...