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Nanoparticle Tracking Analysis (NTA) is an image analysis-based technique that deduces the particle size from the changing position of scattering diffusing particles in a sequence of images. It is shown that a basic understanding of the underlying physical principles largely helps to ...

Nanoparticle Tracking Analysis (NTA) provides direct and real time visualization, sizing, and counting of particulate materials between 10 nm and 1 μm in liquid suspension. The technique works on a particle-by-particle basis, relating the degree of movement under Brownian motion to the ...

In recent years, the near-infrared fluorescence (NIRF) labeled iron nanoparticles were synthesized and applied to labeling human cells for monitoring the engraftment process, imaging tumors, testing intracellular molecular environment surrounding the nanoparticles, a ...

Hydrophilic semiconductor nanoparticles are very attractive for various biological applications, such as in optical sensing, tracing, and imaging of biological molecules-of-interest, because of their broad excitation wavelength, tunable emission wavelength, strong p ...

Here, we describe typical methods and provide detailed experimental protocols for synthesizing and processing various semiconductor nanoparticles which have potential application in biology and medicine. These include synthesis of binary semiconductor nanoparticl ...

The interest in quantum dots (QD) and a number of reported life sciences applications increased dramatically over the last decade. The popularity of QDs stems from better photostability, higher extinction �co-efficients, and unique optical properties such as superior light absorp ...

Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, �cartilage, adipose, and muscle cells. Adult derived MSCs are being actively investigated because of their potential to be utilized for therapeutic cell-based transplantation. Me ...

Quantum dots (QDs) are semiconductor nanocrystals with unique photo-physical properties. QDs are brightly fluorescent and photostable, and therefore represent a novel class of fluorescence probes for bio-imaging and cell monitoring. There are different techniques for label ...

Immunocytochemistry and histochemistry are two most valuable immunochemistry techniques routinely used in biological laboratories. These techniques rely on the use of antibodies to label epitopes of interest in cells. At present, there is a wide range of commercially available o ...

Increased superoxide (O2 •−) and nitric oxide (NO) production is a key mechanism of mitochondrial dysfunction in myocardial ischemia/reperfusion injury. In the complex II, oxidative impairment, decreased protein S-glutathionylation, and increased protein tyrosine nitrat ...

We describe various complementary techniques to achieve multidimensional mitochondrial proteome fractionation and analysis. Previously described methods for 2D-DIGE/mass spectrometry and 1D-SDS-PAGE/Western techniques and protein complex analysis by BN-PAGE/ ...

Two-dimensional HPLC coupled with tandem MS (MS/MS) has become a mainstream technique in the shotgun proteomics for large-scale identification of proteins from biological samples. This powerful technology provides speed, sensitivity, and dynamic range which are essential to pr ...

The suction blister model was originally developed for the separation of viable epidermis from dermis by Kiistala (1). Since its development, its use has been expanded and applied to several other applications. One new application assesses the collagen synthesis rate in the human skin in vivo ...

The incidence of fire deaths in the United States has decreased from 2.5/100,000 population in 1983 to 1.7/100,000 in 1993 (1). In the last century, tremendous strides have occurred in the acute resuscitation of burns, permitting burn clinicians and researchers to concentrate on improving fun ...

The purpose of this chapter is to review the changes in wound healing that occur in the aged. Unlike pathological conditions such as infection or diabetes as a cause for impaired wound repair, aging may simply reduce the speed at which an individual heals. Thus, a goal of this review is to assess whether aging ...

Wound healing is a highly ordered and well coordinated process that involves inflammation, cell proliferation, matrix deposition, and tissue remodeling (1). During the past few years, a series of candidate key players in the wound-healing scenario have been identified. These include not ...

Diabetes mellitus is a major cause of impaired tissue repair. Patients with this disease not only have a propensity to develop wounds, but when they do, they tend to have difficulty healing those wounds. Simple wounds often become chronic and infectious wound complications are not uncommon. Un ...

The development of strategies for the treatment of angiogenesis-dependent diseases has been greatly aided by the development of in vivo models of angiogenesis (1). These bioassays provide investigators with tools to visualize vessel architecture and function and to analyze and man ...

This chapter is devoted to a fascinating topic in molecular biology—scarless fetal wound repair. Our understanding of this phenomenon may one day allow us to manipulate the adult wound-healing process to recapitulate the scarless phenotype. To achieve this goal, we must first understand ...

The lining of the organs within the peritoneal cavity consists of a single layer of mesothelial cells with a minimum of underlying connective tissue. This same cellular structure covers the luminal surface of the abdominal wall musculature. Together these mesothelial layers serve as a smo ...