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Essential hypertension represents a cluster of genetically determined physiological abnormalities that is influenced by environmental factors and the individual’s lifestyle. Essential hypertension is a major public health challenge because it affects one in every three a ...

G protein-coupled receptors (GPCRs) comprise the largest family of membrane-spanning proteins in humans with approximately 800 members. GPCRs carry out an extensive array of biological functions and are privileged drug targets. Exon sequencing identified several disease-ca ...

Cardiovascular diseases are the most common cause of death worldwide, and despite advances in treatments, mortality rates still remain elevated. Heart failure prevalence is increasing and accounts for half a million deaths per year in the USA. Thus, there is an urgent need to develop new thera ...

G protein-coupled receptors (GPCRs) represent the largest family of cell surface receptors that are involved in regulating several physiological and behavioral responses in organisms. Indeed, over half of all the approved drugs on the market target GPCRs. Over the past 20 years, several l ...

Leucine-rich repeat-containing G protein-coupled receptors (LGRs) are type A rhodopsin-like GPCRs that include the glycoprotein pituitary hormone receptors for the thyroid-stimulating, follicle-stimulating, and luteinizing hormones (TSH, FSH, and LH); a pair of receptors ...

Adult neurogenesis is the process of creating new brain cells during adulthood. This involves several stages including proliferation, migration, differentiation, integration, and survival. G-protein-coupled receptors (GPCRs) regulate this process in both neurogenic re ...

Cancer at its root is a genetic disease brought on by genomic alterations that lead to uncontrolled cell division, metastasis, and enhanced cell survival. The study of genomics can address many of the genetic abnormalities associated with cancer and represents a rapidly progressing field. ...

Metabolic pathways and bioenergetics were described in great detail over half a century ago, and during the past decade a resurgence in integrating these cellular processes with other biological properties of the cell, including growth control, protein kinase cascade signaling, cell ...

The azoxymethane (AOM) plus dextran sulfate sodium (DSS)-induced mouse colon carcinogenesis model is a fascinating tool for investigating the pathogenesis and chemoprevention of colitis-associated colorectal cancer. In this two-stage mouse colon cancer model, tumorigen ...

Mouse lung tumor models are widely used in lung cancer chemopreventive studies. Lung cancer is a heterogeneous disease histologically classified as small-cell lung carcinoma (SCLC) and non-small-cell lung carcinoma (NSCLC), which is usually divided into adenocarcinoma, squamo ...

Our laboratory has f cancer prevention using freeze-dried berries, mainly black raspberries, for more than two decades. Berries contain many known agents with chemopreventive potential including certain vitamins, minerals, simple and complex polyphenols, phytosterols, and v ...

Prostate cancer is now diagnosed mostly at an early stage, and therefore, chemopreventive strategies could be useful to prevent further progression of the disease and to reduce the morbidity and mortality due to this malignancy. Here, we have described methods (immunoblotting, immuno ...

Cancer chemoprevention with natural phytochemical compounds is an emerging strategy to prevent, impede, delay, or cure cancer. This chapter reviews the basic methods used to study the cancer chemopreventive potential of dietary phytochemicals acting by activating the transcri ...

Carcinogenesis is a long-term, multifactorial, and multistep process. Dietary phytochemicals can play a significant role in cancer prevention. In this chapter, we describe common protocols to study the role of phytochemicals in chemoprevention and divide the protocols into two typ ...

Targeting specific and multiple cancer genes, signaling proteins, and transcription factors to prevent cancer is now considered to be the most effective means to prevent cancer. Proteins that bind to a specific DNA gene sequence and act to initiate transcription of the distinct protein ge ...

Chemoprevention represents an important part of cancer medicine’s future. The identification of chemopreventive agents holds tremendous promise for reducing the burden of cancer. Currently, 13 agents are FDA approved for the treatment of precancerous lesions or to reduce the risk of ...

This is an exciting era in the development and cross-disciplinary use of new imaging technologies, including single-photon and multiphoton laser scanning microscopy; second- and third-harmonic generation imaging; coherent anti-Raman stokes imaging (CARS); and live-cell, who ...

DNA adduct measurements provide valuable information about DNA damage associated with exposure to specific genotoxicants. N 2-Ethylidene-dGuo, the major DNA adduct formed upon reaction of acetaldehyde with DNA, has been used to investigate mechanisms of alcohol carcinogenes ...

Primary keratinocytes are harvested from the dorsal skin of 7-week-old mice. Euthanized mice are clipped and cleaned with serial washes in povidone iodine and ethanol solutions. The dorsal skin is removed and treated with a mild 32 �C trypsinization to detach the dermis from the epidermis. Ker ...

Metabolomics is a comprehensive profiling tool used to identify qualitatively and quantitatively all the metabolites present in a biological system. As the number of metabolites in a living being is assumed to be around 30,000, it is necessary to use an adequate extraction procedure and a good ...