相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg
SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated(产品说明)
蛋白名称:SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation N501Y) (Arg319-Phe541) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The purified protein was biotinylated in vitro. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.7) which emerged in the UK.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated (Cat: 40592-V49H2-B), the EC50 of SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated(Cat: 40592-V49H2-B) is 3-18 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ser
蛋白分子量:The recombinant SARS-CoV-2 Spike RBD consists of 250 amino acids and predicts a molecular mass of 28.49 kDa. As a result of glycosylation, it migrates as an approximately 37.69 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Arg319-Phe541
蛋白标签:C-His-AVI
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称:SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation N501Y) (Arg319-Phe541) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The purified protein was biotinylated in vitro. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.7) which emerged in the UK.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated (Cat: 40592-V49H2-B), the EC50 of SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated(Cat: 40592-V49H2-B) is 3-18 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ser
蛋白分子量:The recombinant SARS-CoV-2 Spike RBD consists of 250 amino acids and predicts a molecular mass of 28.49 kDa. As a result of glycosylation, it migrates as an approximately 37.69 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Arg319-Phe541
蛋白标签:C-His-AVI
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
观察到小鼠出现炎症和不良反应。检测分析灭活疫苗的免疫原性显示,小鼠体内可被诱导产生针对SARS-CoV-2的S蛋白和RBD特异性的IgG,且针对RBD特异性的IgG占S蛋白抗体反应高达50%,提示RBD是新冠病毒灭活疫苗的主要免疫原。 2. 定点插入人源化hACE2小鼠模型中检院实验动物资源研究所主导的研究团队首次报道成功构建定点插入hACE2-KI/NIFDC小鼠模型。该人源化小鼠构建策略是将hACE2基因定点插入小鼠mAce2基因启动子之下,并将tdTomato基因插入到hACE2基因的下游
技术资料暂无技术资料 索取技术资料
义翘神州 新冠RBD S蛋白 生物素化 His&AVI标签 RBD S蛋白
¥4520










