SARS-CoV-2 Spike RBD (N501Y) P

SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated（产品说明）
蛋白名称：SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation N501Y) (Arg319-Phe541) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The purified protein was biotinylated in vitro. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.7) which emerged in the UK.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated (Cat: 40592-V49H2-B), the EC50 of SARS-CoV-2 Spike RBD (N501Y) Protein (His & AVI Tag), Biotinylated(Cat: 40592-V49H2-B) is 3-18 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Ser
蛋白分子量：The recombinant SARS-CoV-2 Spike RBD consists of 250 amino acids and predicts a molecular mass of 28.49 kDa. As a result of glycosylation, it migrates as an approximately 37.69 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
蛋白标签：C-His-AVI
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.