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文献和实验Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
, however, with much improved sensitivity allowing investigation of a greater range of cell types. In the early 1980s , the “Null Point method” was introduced (4 ), which involved addition of a metallochromic calcium indicator (Arsenazo III) to cells permeabilized
Assessment of Intracellular Calcium in Cerebral Artery Myocytes
frequency and elevated global [Ca2+ ]i in cerebral artery myocytes using a rabbit model of subarachnoid hemorrhage. Here, we describe and discuss laboratory methods and procedures using the fluorescent Ca2+ indicator dyes fura-2 and fluo-4 to assess global
Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kDa protein absorbs blue light from a photoprotein that is activated by calcium and emits green
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