SV40 T-Ag-derived Nuclear Loca

核定位信号(nuclear localization signal, NLS)

核定位信号是另一种形式的信号肽, 可位于多肽序列的任何部分。一般含有 4～8个氨基酸, 且没有专一性, 作用是帮助亲核蛋白进入细胞核。入核信号与导肽的区别在于: ①由含水的核孔通道来鉴别; ②入核信号是蛋白质的永久性部分,在引导入核过程中,并不被切除, 可以反复使用, 有利于细胞分裂后核蛋白重新入核。 有多种类型的核定位信号，这些信号都具有一个带正电荷的肽核心。第一个被确定的NLS序列的蛋白质是SV40的T抗原(MW=92kDa), 它在细胞质中合成后很快积累在细胞

Coupling of Nuclear Localization Signals to Plasmid DNA

This chapter focuses on a methodology for covalently associating nuclear localization signal (NLS) peptides to DNA, in which cationic NLS peptides are covalently bound to plasmid DNA by photoactivation. Described here are the synthesis

细胞渗透蛋白（CPP）

-Gal by adenovirus in vivo and in vitro [38]. Furthermore, the linking of the SV40 nuclear localization signal (NLS) peptide sequence (PKKKRKV) via PNA to a reporter gene that carried the vector, transfecting cells and polyethylenimine (PEI) resulted