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- 英文名：
Solulink A-9401-001 RapidDirect Primary Antibody polyHRP Western Blot Kit
- 供应商：
北京诺博莱德科技有限公司
Solulink抗体标签、抗体-寡肽标签、生物素标签、磁珠及凝胶
Solulink ( 美国 )
Solulink — 生物分子标记的领跑者；Solulink公司致力于发展高性能的生物分子连接产品，他们不断的努力帮助你保持在生物科学研究的最前沿。
Solulink是第一家提供包括纯化的完整抗体标记试剂盒的公司。
开发有可用于任何含有氨基的生物分子标记的ChromaLink™抗体标签试剂盒（ChromaLink™ Antibody Labeling Kit），和One-Shot（单标签）系列试剂盒。
Solulink — 生物分子标记的领跑者
我们是第一家提供包括纯化的完整抗体标记试剂盒的公司，而且我们正在开发更多令人振奋的新结合试剂盒。这一切都基于一个成功的产品理念：易于使用，价格便宜。
Solulink公司的产品包括：抗体标签、抗体-寡肽标签、生物素标签、磁珠及凝胶。
我们北京诺博莱德科技有限公司作为Solulink公司的经销商为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。
Solulink品牌部分产品货号：
| 产品品牌 | 产品编号 | 产品名称 | 规格 |
| Solulink | A-9001-006 | R-PE-Antibody All-in-One Conjugation Kit | 2 x 100 ug |
| Solulink | A-9002-001 | HRP-Antibody All-In-One Conjugation Kit | 2 x 100 ug |
| Solulink | A-9105-001 | AP-Antibody All-In-One Conjugation Kit | 2 x 100 ug |
| Solulink | A-9202-001 | Antibody-Oligonucleotide All-in-One Conjugation Kit | 100 ug |
| Solulink | A-9302-001 | HRP-Antibody All-in-One Conjugation Kit Large Scale | 5 mg |
| Solulink | A-9401-001 | RapidDirect Primary Antibody polyHRP Western Blot Kit | 10–20 blots |
| Solulink | A-9402-001 | RapidDirect Primary Antibody polyHRP Leling Kit | 1 x 100 ug |
| Solulink | A-9403-001A | RapidDirect Primary Antibody polyHRP IP/Western Blot Kit (with goat anti-mouse antibody beads) | 10–20 blots |
| Solulink | A-9403-001B | RapidDirect Primary Antibody polyHRP IP/Western Blot Kit (with goat anti-rbit antibody beads) | 10–20 blots |
| Solulink | A-9403-001C | RapidDirect Primary Antibody polyHRP IP/Western Blot Kit (with rbit anti-goat antibody beads) | 10–20 blots |
| Solulink | A-9404-001 | NanoLink BeadLink Kit | 100 ug of |
| Solulink | A-9404-002 | MagnaLink BeadLink Kit | 100 ug of |
| Solulink | B-1001-010 | ChromaLink Biotin Leling Reagent (DMF Soluble) | 10 mg |
| Solulink | B-1001-105 | ChromaLink Biotin Leling Reagent (DMF Soluble) | 5 x 1.0 mg |
| Solulink | B-1002-025 | Biotin/PEG4/Succinimidyl Ester | 25 mg |
| Solulink | -1122 | Biotin/PEG4/Succinimidyl Ester | 10 x 2.0 mg |
| Solulink | B-1005-025 | Biotin Succinimidyl Ester | 25 mg |
| Solulink | B-1005-120 | Biotin Succinimidyl Ester | 10 mg |
| Solulink | B-1007-105 | ChromaLink Biotin Leling Reagent (Water Soluble) | 5 x 1.0 mg |
| Solulink | B-1007-110 | ChromaLink Biotin Leling Reagent (Water Soluble) | 10 mg |
| Solulink | B-1012-010 | ChromaLink Biotin Maleimide Reagent | 10 mg |
| Solulink | B-1014-005 | ChromaLink Digoxigenin Leling Reagent | 0.5 mg |
| Solulink | B-1014-505 | ChromaLink Digoxigenin Leling Reagent | 5 x 0.5 mg |
| Solulink | B-9007-009K | ChromaLink One-Shot Antibody Biotinylation Kit | 100 ug |
| Solulink | B-9007-105K | ChromaLink Biotin Leling Kit | 5 rxns |
| Solulink | B-9014-009K | ChromaLink Digoxigenin One-Shot Antibody Leling Kit | 100 ug |
| Solulink | F-9001-009K | Fluorescein One-Shot Antibody Leling Kit | 2 x 100 ug |
| Solulink | HL-1002-025 | HyNic Silane | 25 mg |
| Solulink | HL-1004-025 | 4FB Silane | 25 mg |
| Solulink | M-1000-001 | NanoLink Amino Magnetic Beads (0.8 um) | 1 mL |
| Solulink | M-1001-010 | NanoLink 4FB Magnetic Beads (0.8 um) | 1 mL |
| Solulink | M-1002-010 | NanoLink Streptavidin Magnetic Beads (0.8 um) | 1 mL |
| Solulink | M-1003-010 | MagnaLink Streptavidin Magnetic Beads (2.8 um) | 1 mL |
| Solulink | M-1004-010 | MagnaLink 4FB Magnetic Beads (2.8 um) | 1 mL |
| Solulink | M-1005-010 | MagnaLink Amino Magnetic Beads (2.8 um) | 1 mL |
| Solulink | N-1000 | Streptavidin Agarose Ultra Performance | 10 mL |
| Solulink | N-1000-002 | Streptavidin Agarose Ultra Performance | 2 mL |
| Solulink | N-1000-005 | Streptavidin Agarose Ultra Performance | 5 mL |
| Solulink | N-1000-010 | Streptavidin Agarose Ultra Performance | 10 mL |
| Solulink | P-9002-002 | R-PE-Antibody Conjugation Kit | 1 x 1.0 mg |
| Solulink | P-9903-001 | APC-Antibody Conjugation Kit | 1 x 1.0 mg |
| Solulink | S-1001-010 | SHNH (HyNic for technitium-99M leling) | 10 mg |
| Solulink | S-1002-010 | S-HyNic Crosslinker (DMF Soluble) | 10 mg |
| Solulink | S-1002-105 | S-HyNic Crosslinker (DMF Soluble) | 5 x 1.0 mg |
| Solulink | S-1003-010 | SHTH (heat-lile version of S-HyNic) | 10 mg |
| Solulink | 04-010 | S-4FB Crosslinker (DMF Soluble) | 10 mg |
| Solulink | S-1004-105 | S-4FB Crosslinker (DMF Soluble) | 5 x 1.0 mg |
| Solulink | S-1005-250 | 4FB-Phosphoramidite | 250 mg |
| Solulink | S-1007-025 | C6-S-4FB Crosslinker | 25 mg |
| Solulink | S-1007-105 | C6-S-4FB Crosslinker | 5 x 1.0 mg |
| Solulink | S-1008-010 | Sulfo S-4FB Crosslinker (Water Soluble) | 10 mg |
| Solulink | S-1008-105 | Sulfo S-4FB Crosslinker (Water Soluble) | 5 x 1.0 mg |
| Solulink | S-1009-010 | MHPH (Maleimide HyNic) Crosslinker | 10 mg |
| Solulink | S-1010-010 | C6-S-HyNic Crosslinker | 10 mg |
| Solulink | S-1010-025 | C6-S-HyNic Crosslinker | 25 mg |
| Solulink | S-1011-010 | Sulfo S-HyNic Crosslinker (Water Soluble) | 10 mg |
| Solulink | S-1011-105 | Sulfo S-HyNic Crosslinker (Water Soluble) | 5 x 1.0 mg |
| Solulink | S-1034-010 | PEG4/PFB Long Chain Crosslinker (analog of 4-FB) | 10 mg |
| Solulink | S-1035-105 | MTFB (Maleimide 4FB) Crosslinker | 5 x 1.0 mg |
| Solulink | S-1037-010 | S-SS-4FB Cleavle Crosslinker | 10 mg |
| Solulink | S-1037-105 | S-SS-4FB Cleavle Crosslinker | 5 x 1 mg |
| Solulink | S-2002-100 | 2-Hydrazinopyridine.dihydrochloride (to quantify 4FB-modified biomolecules) | 100 mg |
| Solulink | S-2003-100 | 6-HNA (6-hydrazinonicotinic acid) (used in peptide synthesis) | 100 mg |
| Solulink | S-2005-100 | 2-Sulfobenzaldehyde (to quantify HyNic-modified biomolecules) | 100 mg |
| Solulink | S-2006-105 | TurboLink Catalyst Buffer (10X) | 1.5 mL |
| Solulink | S-3001-100 | FMOC-HNA-Osu (incorporates FMOC-protected HyNic on N-terminus) | 100 mg |
| Solulink | S-3002-100 | 6-BOC-HNA-OSu (incorporates BOC-protected HyNic to N-term or internal Lys) | 100 mg |
| Solulink | S-3002-250 | 6-BOC-HNA-OSu (incorporates BOC-protected HyNic to N-term or internal Lys) | 250 mg |
| Solulink | S-3003-100 | 6-BOC-HNA (incorporates BOC-protected HyNic to N-term or internal Lys) | 100 mg |
| Solulink | S-3003-500 | 6-BOC-HNA (incorporates BOC-protected HyNic to N-term or internal Lys) | 500 mg |
| Solulink | S-3004-100 | 6-FMOC-HNA (incorporates FMOC protected HyNic to N-term or internal Lys) | 100 mg |
| Solulink | S-3004-250 | 6-FMOC-HNA (incorporates FMOC protected HyNic to N-term or internal Lys) | 250 mg |
| Solulink | S-3004-500 | 6-FMOC-HNA (incorporates FMOC protected HyNic to N-term or internal Lys) | 500 mg |
| Solulink | S-3034-100 | FMOC Lysine-e-(6-BOC-HyNic)OH (incorporates HyNic on C-term or internal positions) | 100 mg |
| Solulink | S-3034-500 | FMOC Lysine-e-(6-BOC-HyNic)OH (incorporates HyNic on C-term or internal positions) | 500 mg |
| Solulink | S-4000-005 | Modification Buffer (10X) | 5 x 1.5 mL |
| Solulink | S-4001-005 | Anhydrous DMF | 5 x 1.5 mL |
| Solulink | S-4002-005 | Conjugation Buffer (10X) | 5 x 1.5 mL |
| Solulink | S-4004-010 | VivaSpin Diafiltration Spin Filters (5K MWCO) | 10 pack |
| Solulink | S-4006-010 | Fluorescein/PEG4/4FB | 10 mg |
| Solulink | S-4008-001 | 4FB Activated R-PE | 1 mg |
| Solulink | S-4008-003 | 4FB Activated R-PE | 3 mg |
| Solulink | S-4009-001 | 4FB Activated APC | 1 mg |
| Solulink | S-4009-003 | 4FB Activated APC | 3 mg |
| Solulink | S-4017-105 | PBS Buffer (10X) | 5 x 1.5 mL |
| Solulink | S-4023-025 | Bead Block Solution | 25 mL |
| Solulink | S-4024-010 | Zeba Desalting Columns (retains salts and small molecules <7 kDa) | 10 pack |
| Solulink | S-4026-105 | MES Buffer (10X) | 5 x 1.5 mL |
| Solulink | S-6001-001 | Magnetic Stand | 1.5 mL |
| Solulink | S-9002-1 | All Purpose Crosslinking Kit | Kit |
| Solulink | S-9010-1 | Protein-Protein Conjugation Kit | Kit |
| Solulink | S-9011-1 | Protein-Oligo Conjugation Kit | Kit |
| Solulink | SP-C001-005 | HyNic-ARG8 | 0.5 mg |
| Solulink | SP-C001-010 | HyNic-ARG8 | 1.0 mg |
| Solulink | SP-C002-005 | TAT-HyNic | 0.5 mg |
| Solulink | SP-C002-010 | TAT-HyNic | 1.0 mg |
| Solulink | SP-E001-005 | 6XHis Tag HyNic | 0.5 mg |
| Solulink | SP-E001-010 | 6XHis Tag HyNic | 1.0 mg |
| Solulink | SP-E002-005 | S Tag HyNic | 0.5 mg |
| Solulink | SP-E002-010 | S Tag HyNic | 1.0 mg |
| Solulink | SP-E003-005 | C-Myc Tag HyNic | 0.5 mg |
| Solulink | SP-E003-010 | C-Myc Tag HyNic | 1.0 mg |
| Solulink | SP-E004-005 | Flag Tag HyNic | 0.5 mg |
| Solulink | SP-E004-010 | Flag Tag HyNic | 1.0 mg |
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文献和实验相关实验
免疫亲和层析法常用于蛋白质等生物大分子的纯化。但你是否想过，一根亲和层析柱子也能用来进行细胞分选，而且还不使用高亲和力抗体？ 在细胞分选中，我们常常利用免疫识别特性来进行，比如流式分选(FACS)和免疫磁分选(MACS)。 免疫细胞的划分是依据表面标志 CD 分子，可以被抗体识别。比如针对 CD4 + T 细胞这一特定类群，表面含有 CD45、CD3 和 CD4 标记。而表达 CD4 就是辅助性 T 细胞的特征。 由此而来，免疫磁分选利用特定抗体偶联磁珠来捕获细胞。细胞与磁珠
其他方法验证更详细的相互作用信息。 图 11：使用抗体进行 Co-IP 与使用 Strep-Tactin®XT 进行pull-down 融合蛋白沉降 pull-down IP 和 Co-IP 通过固定抗体来捕获诱饵蛋白质及其结合分子，而 pull-down 可使用带亲和标签的目的蛋白进行，在体外验证蛋白间的直接相互作用。Pull-down 是通过磁珠/琼脂糖填料固定带有标记的「诱饵」蛋白，从细胞裂解液、纯化蛋白或表达系统中捕获「猎物」蛋白，然后将复合物蛋白分离后进行凝胶或质谱分析，从而确认互作
用的方法之一，是亲和层析法，借助标签和配体的亲和力进行蛋白纯化。纯化的重组蛋白通常能以特异性的方式被洗脱，例如，在 Strep 标签蛋白纯化中，加入生物素（biotin）即可断开 Strep 标签与配体 Strep-Tactin 的作用，达到有效洗脱的目的。得到高纯度有活性的蛋白质应用在多种下游实验中，如研究蛋白质间相互作用等。 那么是否可以以此为基础，将标签配体亲和力加以利用，结合免疫识别特性，用温和的方法得到高纯度的目的细胞呢？ 1、「画龙点睛」的表位标签——Fab-Streps 抗体
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Solulink抗体标签、抗体-寡肽标签、生物素标签、磁珠及凝胶
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