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- 2026年05月29日
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- 文献和实验
- 技术资料
- 保存条件：
-15℃
- 保质期：
24个月
- 库存：
1
- 供应商：
西安百萤生物科技有限公司
产品基本信息
产品名称：Buccutite FOL，马来酰亚胺 [MTAM]
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
外观：固体
分子量：514.54
溶剂：DMSO
产品介绍
Buccutite 交联技术提供了方便、有效的交联方法，以高共轭产率连接两个生物分子。该方法使用一对交联剂：Buccutite MTA 和 Buccutite FOL。 MTA 被添加到一个分子中，而 FOL 被添加到另一个分子中。通过在中性条件下混合 Molecule-1-Buccutite MTA 和 Molecule-2-Buccutite FOL 来引发交联反应。Buccutite MTA 马来酰亚胺 (MTAM) 的使用方式与广泛用于交联蛋白质的 SMCC 相同。MTAM 的一端（通过马来酰亚胺）与还原抗体（通过 TCEP 或 DTT）中发现的半胱氨酸的硫醇 (-SH) 反应。 SMCC 交联需要高浓度的蛋白质。此外，SMCC 修饰的蛋白质极不稳定，通常会自我反应，因为蛋白质通常同时含有胺和硫醇基团，这会导致大量的同交联。 Buccutite 交联反应在极其温和和中性的条件下发生，无需任何催化剂，它比SMCC更加稳定。
参考文献
A ubiquitin switch controls autocatalytic inactivation of the DNA-protein crosslink repair protease SPRTN.
Authors: Zhao, Shubo and Kieser, Anja and Li, Hao-Yi and Reinking, Hannah K and Weickert, Pedro and Euteneuer, Simon and Yaneva, Denitsa and Acampora, Aleida C and Götz, Maximilian J and Feederle, Regina and Stingele, Julian
Journal: Nucleic acids research (2021): 902-915
DNA-protein crosslink proteases in genome stability.
Authors: Ruggiano, Annamaria and Ramadan, Kristijan
Journal: Communications biology (2021): 11
DNA-protein crosslinks are repaired via homologous recombination in mammalian mitochondria.
Authors: Chesner, Lisa N and Essawy, Maram and Warner, Cecilia and Campbell, Colin
Journal: DNA repair (2021): 103026
Emerging roles of Wss1 in the survival of Candida albicans under genotoxic stresses.
Authors: Homchan, Aimorn and Sukted, Juthamas and Matangkasombut, Oranart and Pakotiprapha, Danaya
Journal: Current genetics (2021): 99-105
Previously unknown type of protein crosslink discovered.
Authors: Fass, Deborah and Semenov, Sergey N
Journal: Nature (2021): 343-344
Protein-oligonucleotide conjugates as model substrates for DNA-protein crosslink repair proteases.
Authors: Reinking, Hannah K and Stingele, Julian
Journal: STAR protocols (2021): 100591
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文献和实验参考文献
A ubiquitin switch controls autocatalytic inactivation of the DNA-protein crosslink repair protease SPRTN.
Authors: Zhao, Shubo and Kieser, Anja and Li, Hao-Yi and Reinking, Hannah K and Weickert, Pedro and Euteneuer, Simon and Yaneva, Denitsa and Acampora, Aleida C and Götz, Maximilian J and Feederle, Regina and Stingele, Julian
Journal: Nucleic acids research (2021): 902-915
DNA-protein crosslink proteases in genome stability.
Authors: Ruggiano, Annamaria and Ramadan, Kristijan
Journal: Communications biology (2021): 11
DNA-protein crosslinks are repaired via homologous recombination in mammalian mitochondria.
Authors: Chesner, Lisa N and Essawy, Maram and Warner, Cecilia and Campbell, Colin
Journal: DNA repair (2021): 103026
Emerging roles of Wss1 in the survival of Candida albicans under genotoxic stresses.
Authors: Homchan, Aimorn and Sukted, Juthamas and Matangkasombut, Oranart and Pakotiprapha, Danaya
Journal: Current genetics (2021): 99-105
Previously unknown type of protein crosslink discovered.
Authors: Fass, Deborah and Semenov, Sergey N
Journal: Nature (2021): 343-344
Protein-oligonucleotide conjugates as model substrates for DNA-protein crosslink repair proteases.
Authors: Reinking, Hannah K and Stingele, Julian
Journal: STAR protocols (2021): 100591
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