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文献和实验CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
cover with a 24 X 50 mm coverslip. Incubate for 5 mins, then gently remove coverslip . Add 85 ul of a mix of anti-digoxigenin-rhodamine/FITC Avidin (at 1:200/1:400 in preblock solution; e.g. 2 ul anti-dig rhodamine + 1 ul FITC avidin in 397
Direct Fluorochrome-Labeled DNA Probes for Direct Fluorescent In Situ Hybridization to Chromosomes
(chapter 25 ). Fig. 1. Direct fluorescent double in situ hybridization to a partial metaphase of a hexaploid wheat variety carrying a rye translocation (1B/1R). (A) Bright fluorescence of the rye chromosome arms probed with rhodamine-labeled
DIG (Digoxigenin) labeled RNA probe In situ hybridization protocol
and rehydrated. Incomplete removal of paraffin can cause poor staining of the section. 1) Xylene 2) 100% ethanol 3) 95% ethanol Place the slides in a rack, and perform the following washes: 4) Xylene: 2 x 3 minutes
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