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文献和实验Transfection Complexes Generated with Adenovirus and Polyethylenimine-Condensed DNA
. A versatile method involves biotinylating the adenovirion and linking the plasmid DNA with a streptavidin-polylysine bridge (6 ). Other methods include direct chemical linkage between virus and polylysine (11 –14 ), as well as an enzymatic linkage using
Environment-Responsive Multifunctional Liposomes
conjugate. Under normal conditions, liposome-grafted PEG “shielded” liposome-attached TATp moieties, since the PEG spacer for TATp attachment (PEG(1000)) was shorter than protective PEG(2000). PEGylated liposomes accumulate in targets via the EPR effect
part of the procedure and unfortunately it is the most critical as well. Wash the cells once in sorbitol buffer and suspend in 100-500ul of the same. BE VERY VERY GENTLE! Place on ice. Coat the wells of teflon coated slides with 0.1 polylysine (>400,000 MW
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