DPPE PEG Maleimide, DPPE-PEG-M

Micro- and Mid-Scale Maleimide-Based Conjugation of Cytotoxic Drugs to Antibody Hinge Region Thiols for Tumor Targeting

and stability. Here we elaborate detailed protocols using maleimide-based chemistry for the conjugation to reduce hinge disulfides in antibodies by several cytotoxic drugs. We present a method for the initial characterization of the reduction/alkylation reaction

PEGylation of Antibody Fragments for Half-Life Extension

of polyethylene glycol (PEG) chain to protein using PEGylation technology. The most suitable approach employs PEG-maleimide attachment to cysteines, either to the free hinge cysteine or to C-terminal cysteines involved in interchain disulfide linkage of the heavy

用sephadex G-25脱盐，如何选择缓冲液

3) 请教色谱兄，我现在用纤维素分离纯化蛋白和peg-蛋白，跑完我用page检测了一下，没有完全分开，有一些是一起流出来的，我想知道为什么分离效果不好？我该如何调整阿？加长柱子可行吗？还是要更换介质阿？我用的梯度洗脱。多谢拉！ peg-蛋白要想完全分开是比较困难的,因为PEG修饰的程度不一样,所很难完全分开,不过纤维素分离的效果是要差点,你可以通过延长柱子,降低流速,延长梯度的办法来提高分辨率,不行你可以换琼脂糖系列的,但是我觉得你也可以考虑疏水,这两个方法都可以.此外洗