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文献和实验Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery
lipoplexes with nucleic acids has significant promise as an efficient delivery system in gene therapy. To achieve in vivo stability and long circulation, most lipoplexes are modified with PEG (PEGylation). However, we reported that PEGylated liposomes lose
PEG Preparation of Plasmid DNA
PEG Preparation of Plasmid Plasmid isolated by this procedure can be used routinely
acid, pH4.8. Steps 1. Grow overnight cultures of bacteria containing the plasmid of interest. 2. Pellet bacteria by centrifugation and resuspend in 0.02 culture volumes of Solution I. 3. Add to this suspension 0.04 culture volumes of Solution II
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