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文献和实验called visible isotope-coded affinity tags (VICAT reagents) that contain a protein-tagging reagent for reaction with cysteine SH groups, a visible probe for monitoring the chromatographic behavior of the target peptides, a photo-releasable
Generation and Detection of S-Nitrosothiols
switch method—that allows the detection and purification of S -nitrosylated proteins. The principle behind this technology is the substitution of the NO group by a biotin linker in a three-step procedure. First, the all free thiol groups are blocked
Identification of Thioredoxin Target Disulfides Using Isotope-Coded Affinity Tags
quantification of Trx target disulfides in complex protein extracts is described. The procedure utilizes the isotope-coded affinity tag (ICAT) reagents containing a thiol reactive iodoacetamide group and a biotin affinity tag to target peptides containing reduced
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