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文献和实验DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica Particles
in these specimens, an efficient DNA release and purification method is required. In this chapter, I describe an efficient and straightforward purification and concentration method that uses DNA adsorption to a solid surface of silica particles. Comparative analysis
Silica Suspension: add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl,10 mM Tris 7.5,2.5 mM EDTA,50% Ethanol 6 M NaI note
agitation for aqueous solutions add 1/10 vol 3M NaOAc to ensure pH is less than 7 2.Bind DNA to silica add appropriate volume of silica suspension (binds approx 200 ng of DNA / ul of suspension) and mix well sediment silica matrix by centrifugation
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