天冬氨酸-胱氨酸特异性蛋白酶家族抗体

英文名称Caspase-1 P10中文名称天冬氨酸-胱氨酸特异性蛋白酶家族抗体别    名caspase-1 isoform alpha p10 subunit; CASP 1; CASP1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; ICE; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BC; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme. CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; Caspase-1 subunit p10.  研究领域肿瘤  细胞生物  细胞凋亡  抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, 产品应用WB=1:500-2000 Flow-Cyt=1μg/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.分 子 量10/45kDa细胞定位细胞浆 性    状Liquid浓    度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human Caspase-1 P10:321-404/404 亚    型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed产品介绍This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq].

Function:
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

Subunit:
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

Post-translational modifications:
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Similarity:
Belongs to the peptidase C14A family.
Contains 1 CARD domain.

SWISS:
P29466

Gene ID:
834

Database links:

Entrez Gene: 834 Human

Entrez Gene: 12362 Mouse

Entrez Gene: 25166 Rat

Omim: 147678 Human

SwissProt: P29466 Human

SwissProt: P29452 Mouse

SwissProt: P43527 Rat

Unigene: 2490 Human

Unigene: 1051 Mouse

Unigene: 37508 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Caspase-1是半胱氨酸蛋白中的一种酶，参与细胞凋亡的调控。它主要用于各种良、恶性肿瘤的研究。